Oleaginous yeasts have emerged as a sustainable source of renewable oils for liquid biofuels. However, biodiesel production from them has few constraints with respect to their cell disruption and lipid extraction techniques. The lipid extraction from oleaginous yeasts commonly includes dewatering and drying of cell biomass, which requires energy and time. The aim of this work was to establish a process for the lipid extraction techniques from wet biomass applying acid catalyzed hot water, microwave, rapid ultrasonication-microwave treatment together with conventional Bligh and Dyer method. In the wake of testing all procedures, it was revealed that rapid ultrasonication-microwave treatment has great potential to give high lipid content (70.86 % w/w) on the cell dry weight basis. The lipid profile after treatment showed the presence of appropriate quantities of saturated (10.39 ± 0.15%), monounsaturated (76.55 ± 0.19%) and polyunsaturated fatty acids (11.49 ± 0.23%) which further improves biodiesel quality compared to the rest of methods. To the best of our knowledge, this is the first report of using rapid ultrasonication-microwave treatment for the lipid extraction from wet oleaginous yeast biomass in the literature.

含油酵母已成为液体生物燃料的可再生油的可持续来源。然而，从它们生产生物柴油对其细胞破坏和脂质提取技术几乎没有限制。从油质酵母中提取脂质通常包括脱水和干燥细胞生物质，这需要能量和时间。这项工作的目的是建立一种使用酸催化热水，微波，快速超声-微波处理以及常规Bligh和Dyer方法从湿生物质中提取脂质的工艺。在测试所有程序之后，发现快速超声-微波处理具有以细胞干重为基础产生高脂质含量（70.86％w / w）的巨大潜力。处理后的脂质谱显示存在适量的饱和脂肪酸（10.39±0.15％），单不饱和脂肪酸（76.55±0.19％）和多不饱和脂肪酸（11.49±0.23％），与其余方法相比，它可以进一步改善生物柴油的质量。据我们所知，这是文献中首次使用快速超声-微波处理从湿的含油酵母生物质中提取脂质的首次报道。