Three undescribed bisabolane derivatives, trichaspin, trichaspsides A and B, three undescribed cyclonerane sesquiterpenes, 9-cycloneren-3,7,11-triol, 11-cycloneren-3,7,10-triol, and 7,10-epoxycycloneran-3,11,12-triol, and one undescribed harziane diterpene, 11-hydroxy-9-harzien-3-one, were obtained from the culture of Trichoderma asperellum cf44-2, an endophyte of the marine brown alga Sargassum sp. Their structures and relative configurations were assigned by analysis of 1D/2D NMR and MS data, and their absolute configurations were established by ECD or specific optical rotation data. Trichaspin features an unprecedented ethylated bisabolane skeleton, while trichaspsides A and B represent the first aminoglycosides of bisabolane and norbisabolane sesquiterpenes, respectively. Nine of the compounds were evaluated for inhibition of five marine-derived pathogenic bacteria and toxicity to a marine zooplankton.

三种未描述的双水硼烷衍生物，三叉戟，三萜苷A和B，三种未描述的环戊烷倍半萜，9-环神经烯-3,7,11-三醇，11-环神经烯-3,7,10-三醇和7,10-环氧环neran-3， 11,12-三醇，和一个未描述的二萜harziane，11-羟基-9- harzien -3-酮，从培养得到棘孢木霉cf44-2，海洋褐藻的内生菌马尾藻sp。通过对1D / 2D NMR和MS数据的分析，确定了它们的结构和相对构型，并通过ECD或特定的旋光数据确定了它们的绝对构型。Trichaspin的特征是前所未有的乙基化Bisabolane骨架，而Trichaspsides A和B分别代表Bisabolane和Norbisabolane倍半萜的第一个氨基糖苷。评价了其中的九种化合物对五种海洋来源的病原菌的抑制作用以及对海洋浮游动物的毒性。