Nitric Oxide-Loaded Antimicrobial Polymer for the Synergistic Eradication of Bacterial Biofilm,ACS Macro Letters

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Nitric Oxide-Loaded Antimicrobial Polymer for the Synergistic Eradication of Bacterial Biofilm
ACS Macro Letters ( IF 5.1 ) Pub Date : 2018-05-04 00:00:00 , DOI: 10.1021/acsmacrolett.8b00190
Rashin Namivandi-Zangeneh ₁ , Zahra Sadrearhami ₁ , Ali Bagheri ₁ , Maeva Sauvage-Nguyen _{1,

2} , Kitty Ka Kit Ho ₃ , Naresh Kumar ₃ , Edgar H H Wong ₁ , Cyrille Boyer ₁

Affiliation

Bacterial biofilms are often difficult to treat and represent the main cause of chronic and recurrent infections. In this study, we report the synthesis of a novel antimicrobial/antibiofilm polymer that consists of biocompatible oligoethylene glycol, hydrophobic ethylhexyl, cationic primary amine, and nitric oxide (NO)-releasing functional groups. The NO-loaded polymer has dual-action capability as it can release NO which triggers the dispersion of biofilm, whereas the polymer can induce bacteria cell death via membrane wall disruption. By functionalizing the polymers with NO, we observed a synergistic effect in biofilm dispersal, planktonic and biofilm killing activities against Pseudomonas aeruginosa. The NO-loaded polymer results in 80% reduction in biofilm biomass and kills >99.999% of planktonic and biofilm P. aeruginosa cells within 1 h of treatment at a polymer concentration of 64 μg mL^–1. To achieve this synergistic effect, it is imperative that the NO donors and antimicrobial polymer exist as a single chemical entity, instead of a cocktail physical mixture of two individual components. The excellent antimicrobial/antibiofilm activity of this dual-action polymer suggests the advantages of combination therapy in combating bacterial biofilms.

中文翻译：

负载一氧化氮的抗菌聚合物协同消灭细菌生物膜

细菌生物膜通常难以治疗，是慢性和复发性感染的主要原因。在这项研究中，我们报告了一种新型抗菌/抗菌膜聚合物的合成，该聚合物由生物相容性低聚乙二醇、疏水性乙基己基、阳离子伯胺和一氧化氮 (NO) 释放官能团组成。负载 NO 的聚合物具有双重作用，因为它可以释放 NO，从而触发生物膜的分散，而聚合物可以通过膜壁破坏诱导细菌细胞死亡。通过用 NO 对聚合物进行功能化，我们观察到生物膜分散、浮游生物和生物膜杀灭铜绿假单胞菌活性的协同作用. 负载 NO 的聚合物导致生物膜生物量减少 80%，并在 64 μg mL ^-1的聚合物浓度下处理 1 小时内杀死 > 99.999% 的浮游生物和生物膜铜绿假单胞菌细胞。为了实现这种协同效应，NO 供体和抗菌聚合物必须作为单一化学实体存在，而不是两种单独组分的混合物物理混合物。这种双重作用聚合物的优异抗菌/抗生物膜活性表明联合疗法在对抗细菌生物膜方面的优势。

更新日期：2018-05-04

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