Zebrafish (Danio rerio) is a useful model for understanding biomedical properties of bone and are widely employed in developmental and genetics studies. Here, we have studied the development of zebrafish vertebral bone at the nanoscale from adolescence (6 months), early adulthood (10 months) to mid-life (14 months). Characterization of the bone was conducted using energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM) and Peakforce QNM Atomic force microscopy (AFM) techniques. SEM and AFM revealed a lamellar structure with mineralized collagen fibrils. There was a significant increase in the wall thickness from 6 to 10 months (76%) and 10 months to 14 months (26%), which is positively correlated with nanomechanical behavior. An increase in the Ca/P ratio was found which was also positively correlated with nanomechanical properties. The change in mechanical properties and Ca/P are similar to those expected in humans when transitioning from adolescence to mid-life. We suggest that zebrafish serve as a suitable model for further studies on age-related changes in bone ultrastructure.

斑马鱼（斑马鱼）是了解骨骼生物医学特性的有用模型，已广泛用于发育和遗传学研究中。在这里，我们研究了从青春期（6个月），成年初期（10个月）到中年（14个月）的纳米级斑马鱼椎骨的发育。使用能量色散X射线光谱法（EDX），扫描电子显微镜（SEM）和Peakforce QNM原子力显微镜（AFM）技术对骨骼进行表征。SEM和AFM显示出具有矿化胶原纤维的层状结构。壁厚从6到10个月（76％）和10个月到14个月（26％）显着增加，这与纳米力学行为呈正相关。发现Ca / P比的增加与纳米机械性能也正相关。从青春期过渡到中年时，机械性能和Ca / P的变化与人类预期的相似。我们建议斑马鱼可作为进一步研究年龄相关的骨超微结构变化的合适模型。