Background

As a comprehensive discipline that studies food and nutrition, foodomics requires reliable qualitative and quantitative information about the food proteome component in order to extract new integrative information from the complex multivariable space of omics. This new information is necessary to achieve a higher level of understanding of processes in food science and technology, consequently new functions of food and improved markers of food quality and safety and completely transform concept of food safety.

Scope and approach

We are making an effort to present mass spectrometry (MS) based proteomic approaches that are being utilized in different proteomic studies, not necessarily in the field of foodomics, which are important and have the potential to advance this field. Current analytical capabilities of MS-based proteomics together with sample preparation procedures and quantification strategies, and recent technical developments were presented.

Key findings and conclusions

MS-based proteomics enables the analysis of different aspects of proteins and provides a variety of approaches for reliable quantification of individual proteins and/or food proteome. This is a complex field and its successful implementation requires a dedicated analyst, a thorough design of sample preparation procedure, the selection of an MS technique and approach, an adequate type of mass spectrometer, a thorough data analysis and validation. Improvements in the technology of mass spectrometers are continuously expanding capabilities of MS-based proteomics.

中文翻译：

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基于质谱的蛋白质组学作为食品组学工具，用于食品质量和安全性的研究和保证

背景

作为研究食品和营养的综合学科，食品组学需要有关食品蛋白质组学成分的可靠的定性和定量信息，以便从复杂的组学空间提取新的整合信息。为了使人们对食品科学和技术的过程有更高的了解，因此这些新信息是必需的，因此，新的食品功能以及食品质量和安全性指标的改进以及对食品安全性概念的彻底转变也将是必不可少的。

范围和方法

我们正在努力提出基于质谱（MS）的蛋白质组学方法，这些方法已在不同的蛋白质组学研究中使用，而不必在食品组学领域中应用，这是重要的，并且具有推动该领域发展的潜力。介绍了基于质谱的蛋白质组学的当前分析能力，以及样品制备程序和定量策略，以及最新的技术发展。

主要发现和结论

基于质谱的蛋白质组学能够分析蛋白质的不同方面，并提供各种方法对单个蛋白质和/或食品蛋白质组进行可靠的定量。这是一个复杂的领域，其成功实施需要专门的分析人员，彻底的样品制备程序设计，MS技术和方法的选择，适当类型的质谱仪，彻底的数据分析和验证。质谱仪技术的进步正在不断扩展基于MS的蛋白质组学的功能。