Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO₄·7H₂O, K₂SO₄, and (NH₄)Al(SO₄)₂·12H₂O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool. Acceptable Raman spectra of carotenoids were obtained in the range of 200–4000 cm⁻¹. This detection achievement was related to the mode of illumination and collection of scattered light as well as due to resonance Raman enhancement of carotenoid signals under green excitation. The position of diagnostic Raman carotenoid bands corresponds well to those specific carotenoids produced by a given halophile. To our best knowledge, this is the first study of carotenoids included in the laboratory in crystalline chlorides and sulfates, using a miniature portable Raman spectrometer.

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卤化古细菌Halorubrum sodomense和盐杆菌Halorum和极嗜盐细菌Salinibacter ruber（Bacteroidetes）在氯化物和硫酸盐（NaCl，KCl，MgSO ₄ ·7H ₂ O，K ₂ SO ₄和（NH ₄）Al ）的饱和溶液中的细胞悬浮液（SO ₄）₂ ·12H ₂O）蒸发以在实验室生长的晶体中产生微米级夹杂物。使用带有绿色激发（532 nm）的手持式拉曼光谱仪获得这些粉红色夹杂物的拉曼光谱。该便携式仪器不包含任何微观工具。在200–4000 cm ^-1范围内获得了可接受的类胡萝卜素拉曼光谱。该检测结果与照明模式和散射光的收集以及绿色激发下类胡萝卜素信号的共振拉曼增强有关。诊断性拉曼类胡萝卜素条带的位置与给定的嗜盐菌产生的特定类胡萝卜素非常吻合。据我们所知，这是使用微型便携式拉曼光谱仪对实验室中包括在结晶氯化物和硫酸盐中的类胡萝卜素的首次研究。

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