Selenoprotein W (SelW) is an important member of the avian selenoprotein family. It is well known for its important role in protecting neurons from oxidative stress during neuronal development. D-Amino acid (D-serine), as a neurotransmitter in the central nervous system (CNS), can mediate neurotoxicity. D-Amino acid oxidase (DAAO) is responsible for regulating the D-serine levels in cells. However, the correlation between SelW and DAAO is not clear yet. To investigate the regulations between SelW and DAAO, chicken embryo monolayer neurons were treated with D-serine and/or Se. In this study, we predicted molecular binding between SelW and DAAO. These results showed that the 9–16, 18, 41–47 and 66 residues of SelW could combine with the DAAO, which suggested that chicken SelW might be the target of DAAO. We determined the DAAO activity and the mRNA expression of SelW in in vitro cultured chicken embryo primitive neuron cells. D-Serine influenced the activity of DAAO and, moreover, a significant increase in the mRNA expression of SelW was found in neurons treated with Se. Notably, we also observed changes in the expression of SelW and DAAO when neurons were treated with various concentrations of D-serine and Se. In conclusion, these data suggest that D-serine could regulate the mRNA expression of SelW by interfering with the activity of DAAO in chicken embryo neurons.

中文翻译：

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硒蛋白W作为d-氨基酸氧化酶的分子靶标受鸡神经元中d-氨基酸的 调控

硒蛋白W（SelW）是鸟类硒蛋白家族的重要成员。众所周知，它在神经元发育过程中保护神经元免受氧化应激的重要作用。D-氨基酸（D-丝氨酸）作为中枢神经系统（CNS）中的神经递质，可以介导神经毒性。D-氨基酸氧化酶（DAAO）负责调节细胞中D-丝氨酸的水平。但是，SelW和DAAO之间的相关性尚不清楚。为了研究SelW和DAAO之间的调控，用D处理鸡胚单层神经元-丝氨酸和/或硒。在这项研究中，我们预测了SelW和DAAO之间的分子结合。这些结果表明，SelW的9–16、18、41–47和66残基可以与DAAO结合，这表明鸡肉SelW可能是DAAO的目标。我们确定了体外培养的鸡胚原始神经元细胞的DAAO活性和SelW的mRNA表达。D-丝氨酸影响DAAO的活性，此外，在用Se处理的神经元中发现SelW的mRNA表达显着增加。值得注意的是，当用不同浓度的D-丝氨酸和Se处理神经元时，我们还观察到SelW和DAAO的表达变化。总之，这些数据表明，d-丝氨酸可通过干扰鸡胚神经元中DAAO的活性来调节SelW的mRNA表达。