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A method combining a kit with the Bionic e-Eye for rapid on site detection of diarrhetic shellfish poisoning
Analytical Methods ( IF 2.7 ) Pub Date : 2018-05-02 00:00:00 , DOI: 10.1039/c8ay00676h Xianxin Qiu 1, 2, 3 , Longjie Zhong 4, 5, 6, 7, 8 , Ying Gan 4, 5, 6, 7, 8 , Kaiqi Su 4, 5, 6, 7, 8 , Shihui Hu 1, 2, 3 , Ping Wang 4, 5, 6, 7, 8
Analytical Methods ( IF 2.7 ) Pub Date : 2018-05-02 00:00:00 , DOI: 10.1039/c8ay00676h Xianxin Qiu 1, 2, 3 , Longjie Zhong 4, 5, 6, 7, 8 , Ying Gan 4, 5, 6, 7, 8 , Kaiqi Su 4, 5, 6, 7, 8 , Shihui Hu 1, 2, 3 , Ping Wang 4, 5, 6, 7, 8
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Okadaic acid is a kind of fat-soluble diarrhetic shellfish poison existing extensively in shellfish. The rapid on site detection of shellfish contaminated by the toxin is a serious problem. Herein, a new method combining a kit with the Bionic e-Eye was developed for the rapid detection of okadaic acid on site. After optimization the kit reached a very low limit of detection, 0.19 μg L−1, and the linear detection range was 0.2–10 μg L−1. The cross reaction rate with dinophysistoxins 1 was 23.6%. The kit had a small cross reaction with other kinds of toxin like saxitoxin, gynotoxin 2 & 3 and pectenotoxins 2-b. The recovery range was about 75.78–102.56%, and the CVs were lower than 20%. A correlation analysis was conducted between the Bionic e-Eye and a microplate reader in PBS and shellfish backgrounds. The detection results of the real shellfish samples by the Bionic e-Eye exhibited great consistency with high performance liquid chromatography. The total time taken to perform the detection was less than 90 minutes. Above all, the method integrated kit with the Bionic e-Eye was very suitable for the rapid detection of okadaic acid on site.
中文翻译：
一种将试剂盒与仿生e-Eye结合使用的方法，用于快速现场检测腹泻性贝类中毒
冈田酸是贝类中广泛存在的一种脂溶性腹泻性贝类毒素。快速现场检测被毒素污染的贝类是一个严重的问题。本文中，开发了一种将试剂盒与仿生e-Eye结合的新方法，用于现场快速检测冈田酸。经过优化后，试剂盒的检测限非常低，为0.19μgL -1，线性检测范围为0.2–10μgL -1。与狄诺氏菌毒素1的交叉反应率为23.6％。该试剂盒与其他毒素（如毒毒素，人参毒素2和3和果胶毒素2-b）有较小的交叉反应。恢复范围约为75.78–102.56％，CV低于20％。在PBS和贝类背景中的仿生e-Eye和酶标仪之间进行了相关分析。仿生e-ye对真实贝类样品的检测结果与高效液相色谱法具有很好的一致性。执行检测所需的总时间少于90分钟。最重要的是，与仿生e-Eye集成的方法套件非常适合现场快速检测冈田酸。
更新日期：2018-05-02
中文翻译：
一种将试剂盒与仿生e-Eye结合使用的方法，用于快速现场检测腹泻性贝类中毒
冈田酸是贝类中广泛存在的一种脂溶性腹泻性贝类毒素。快速现场检测被毒素污染的贝类是一个严重的问题。本文中，开发了一种将试剂盒与仿生e-Eye结合的新方法，用于现场快速检测冈田酸。经过优化后，试剂盒的检测限非常低，为0.19μgL -1，线性检测范围为0.2–10μgL -1。与狄诺氏菌毒素1的交叉反应率为23.6％。该试剂盒与其他毒素（如毒毒素，人参毒素2和3和果胶毒素2-b）有较小的交叉反应。恢复范围约为75.78–102.56％，CV低于20％。在PBS和贝类背景中的仿生e-Eye和酶标仪之间进行了相关分析。仿生e-ye对真实贝类样品的检测结果与高效液相色谱法具有很好的一致性。执行检测所需的总时间少于90分钟。最重要的是，与仿生e-Eye集成的方法套件非常适合现场快速检测冈田酸。
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