Amyloid-beta oligomers (AβΟ) are considered to be reliable biomarkers for the diagnosis of Alzheimer’ disease (AD), and the cellular prion protein (PrP^C) is identified as a receptor for AβO. We demonstrated a label and antibody-free electrochemical biosensor for the selective detection of AβO using an electrically conductive poly(pyrrole-2-carboxylic acid) (PPyCOOH) linking agent and PrP^C receptor. In the fabrication of the biosensor, each step was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The developed PrPc/PPyCOOH biosensor exhibited extremely low detection limit of 10⁻⁴ pM with high sensitivity which is desirable for the early diagnosis of AD. The sensing performance was further confirmed by the mice infected with AD. The proposed sensor emerged as a promising tool for the early detection of AβO.

淀粉样蛋白β寡聚体（AβΟ）被认为是阿尔茨海默病（AD）的诊断生物标志物可靠，并且蜂窝朊病毒蛋白（PrP的^Ç）被识别为用于AβO的受体。我们展示了一种使用无标签和无抗体的电化学生物传感器，该传感器可使用导电的聚（吡咯-2-羧酸）（PPyCOOH）连接剂和PrP ^C受体选择性检测AβO 。在生物传感器的制造中，每个步骤均通过电化学阻抗谱和循环伏安法进行表征。研发的PrPc / PPyCOOH生物传感器的检出限极低，为10 ^-4具有高灵敏度的pM，对于AD的早期诊断是理想的。感染AD的小鼠进一步证实了感测性能。提出的传感器成为早期检测AβO的有前途的工具。