A new rapid method for the detection of hydrogen sulfide from pathogenic bacteria is reported. The developed method, static headspace – multi-capillary column - gas chromatography - ion mobility spectrometry (SHS-MCC-GC-IMS), has been applied to detect hydrogen sulfide evolution from 61 bacteria. The developed method has been compared against a standard triple sugar iron (TSI) agar approach, and a modified single sugar iron (SSI) agar approach. Hydrogen sulfide detection by SHS-MCC-GC-IMS using an initial inoculum of 1–1.5 × 10⁵ CFU/mL can be achieved within 6 h, after incubation at 37 °C, with a limit of detection of 1.6 ng/mL. Data for the standard agar method against the new instrumental approach, and the modified agar method against the new instrumental approach, are compared. The specificity for the new method compared against the standard method and the modified agar approach across all 61 strains was 85.2% and 88.5% respectively, and 86.7% and 91.3% across the 23 Salmonella strains tested.

报道了一种新的快速检测病原细菌中硫化氢的方法。所开发的方法，静态顶空–多毛细管柱–气相色谱仪-离子迁移谱仪（SHS-MCC-GC-IMS），已用于检测61种细菌中硫化氢的释放。已将开发的方法与标准三糖铁（TSI）琼脂方法和改良的单糖铁（SSI）琼脂方法进行了比较。通过SHS-MCC-GC-IMS使用初始接种量为1-1.5×10 ^5的硫化氢检测 在37°C孵育后6小时内可达到CFU / mL，检测限为1.6 ng / mL。比较了针对新仪器方法的标准琼脂方法和针对新仪器方法的改良琼脂方法的数据。与标准方法和改良琼脂方法相比，新方法在所有61株菌株中的特异性分别为85.2％和88.5％，在23株沙门氏菌菌株中分别为86.7％和91.3％。