Fluorescence Polarization Control for On–Off Switching of Single Molecules at Cryogenic Temperatures,Small Methods

当前位置： X-MOL 学术 › Small Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Fluorescence Polarization Control for On–Off Switching of Single Molecules at Cryogenic Temperatures
Small Methods ( IF 10.7 ) Pub Date : 2018-04-30 , DOI: 10.1002/smtd.201700323
Christiaan N. Hulleman ₁ , Maximiliaan Huisman ₂ , Robert J. Moerland ₁ , David Grünwald ₂ , Sjoerd Stallinga ₁ , Bernd Rieger ₁

Affiliation

Light microscopy, allowing sub‐diffraction‐limited resolution, has been among the fastest developing techniques at the interface of biology, chemistry, and physics. Intriguingly no theoretical limit exists on how far the underlying measurement uncertainty can be lowered. In particular data fusion of large amounts of images can reduce the measurement error to match the resolution of structural methods like cryo‐electron microscopy. Fluorescence, although reliant on a reporter molecule and therefore not the first choice to obtain ultraresolution structures, brings highly specific labeling of molecules in a large assembly to the table and inherently allows the detection of multiple colors, which enables the interrogation of multiple molecular species at the same time in the same sample. Here, the problems to be solved in the coming years, with the aim of higher resolution, are discussed, and what polarization depletion of fluorescence at cryogenic temperatures can contribute for fluorescence imaging of biological samples, like whole cells, is described.

中文翻译：

低温下单分子通断转换的荧光偏振控制

光学显微镜允许亚衍射极限分辨率，是生物学，化学和物理领域发展最快的技术之一。有趣的是，对于基本的测量不确定度可以降低多大程度，没有理论上的限制。特别是大量图像的数据融合可以减少测量误差，以匹配诸如冷冻电子显微镜之类的结构方法的分辨率。荧光虽然依赖于报告分子，因此不是获得超分辨率结构的首选，但荧光可以将大型装配体中的分子高度特异性地标记到工作台上，并固有地允许检测多种颜色，从而可以在多个位置查询多种分子。在同一样本中同时进行。在这里，未来几年要解决的问题，

更新日期：2018-04-30

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文