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Comparative analysis of the cellular entry of polystyrene and gold nanoparticles using the freeze concentration method†
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-04-30 00:00:00 , DOI: 10.1039/c8bm00206a Sana Ahmed 1, 2, 3, 4 , Koyo Okuma 1, 2, 3, 4 , Kazuaki Matsumura 1, 2, 3, 4
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-04-30 00:00:00 , DOI: 10.1039/c8bm00206a Sana Ahmed 1, 2, 3, 4 , Koyo Okuma 1, 2, 3, 4 , Kazuaki Matsumura 1, 2, 3, 4
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Despite advances in nanoparticle delivery, established physical approaches, such as electroporation and sonication, result in cell damage, limiting their practical applications. In this study, we proposed a unique freeze concentration-based technique and evaluated the efficacy of the method using two types of nanoparticles: citrate-capped gold nanoparticles and carboxylated polystyrene nanoparticles. We further compared the internalisation behaviour of particles of various sizes with and without freezing. Confocal microscopic images showed that the uptake efficacy of 50 nm nanomaterials was greater than that of 100 nm particles. Polystyrene nanoparticles of 50 nm size had more favourable adsorption and internalisation behaviours compared to those of gold nanoparticles after freeze concentration. We also examined the possible endocytic pathways involved in the uptake of gold and polystyrene nanoparticles, and found that the route differed between non-frozen and frozen conditions. Overall, we determined the influence of the freeze concentration strategy on both nanomaterial internalisation and the endocytic uptake pathway. Our findings provide a mechanistic understanding of the internalisation of nanoparticles using a freezing approach and thereby contribute to further developments in nanotherapeutic applications.
中文翻译：
冷冻浓缩法对聚苯乙烯和金纳米颗粒进入细胞的比较分析†
尽管在纳米颗粒递送方面取得了进展，但是已建立的物理方法，例如电穿孔和超声处理，会导致细胞损伤，从而限制了它们的实际应用。在这项研究中，我们提出了一种独特的基于冷冻浓缩的技术，并使用两种类型的纳米颗粒评估了该方法的有效性：柠檬酸盐封端的金纳米颗粒和羧化聚苯乙烯纳米颗粒。我们进一步比较了具有和不具有冻结的各种尺寸的颗粒的内在化行为。共聚焦显微镜图像显示，50 nm纳米材料的吸收效率大于100 nm颗粒的吸收效率。冷冻浓缩后，与金纳米颗粒相比，尺寸为50 nm的聚苯乙烯纳米颗粒具有更有利的吸附和内化行为。我们还检查了金和聚苯乙烯纳米颗粒吸收可能涉及的内吞途径，并发现在非冷冻和冷冻条件下该途径是不同的。总的来说，我们确定了冷冻浓缩策略对纳米材料内在化和内吞摄取途径的影响。我们的发现提供了使用冷冻方法对纳米粒子内部化的机械理解，从而为纳米治疗应用的进一步发展做出了贡献。
更新日期：2018-04-30
中文翻译：
冷冻浓缩法对聚苯乙烯和金纳米颗粒进入细胞的比较分析†
尽管在纳米颗粒递送方面取得了进展，但是已建立的物理方法，例如电穿孔和超声处理，会导致细胞损伤，从而限制了它们的实际应用。在这项研究中，我们提出了一种独特的基于冷冻浓缩的技术，并使用两种类型的纳米颗粒评估了该方法的有效性：柠檬酸盐封端的金纳米颗粒和羧化聚苯乙烯纳米颗粒。我们进一步比较了具有和不具有冻结的各种尺寸的颗粒的内在化行为。共聚焦显微镜图像显示，50 nm纳米材料的吸收效率大于100 nm颗粒的吸收效率。冷冻浓缩后，与金纳米颗粒相比，尺寸为50 nm的聚苯乙烯纳米颗粒具有更有利的吸附和内化行为。我们还检查了金和聚苯乙烯纳米颗粒吸收可能涉及的内吞途径，并发现在非冷冻和冷冻条件下该途径是不同的。总的来说，我们确定了冷冻浓缩策略对纳米材料内在化和内吞摄取途径的影响。我们的发现提供了使用冷冻方法对纳米粒子内部化的机械理解，从而为纳米治疗应用的进一步发展做出了贡献。
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