Quantitative Biodetection of Anticancer Drug Rituxan with DNA Biosensor Modified PAMAM Dendrimer/Reduced Graphene Oxide Nanocomposite,Electroanalysis

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Quantitative Biodetection of Anticancer Drug Rituxan with DNA Biosensor Modified PAMAM Dendrimer/Reduced Graphene Oxide Nanocomposite
Electroanalysis ( IF 2.7 ) Pub Date : 2018-03-23 , DOI: 10.1002/elan.201800014
Behnaz Hatamluyi ₁ , Zarrin Es'haghi ₁

Affiliation

PAMAM dendrimer/reduced graphene oxide nanocomposite modified pencil graphite electrode (PAMAM/RGO/PGE) was used to fabricate an electrochemical DNA biosensor for determination of Rituxan (RTX) at low concentrations, for the first time. The fabricated biosensor was characterized with FE‐SEM, EIS, and CV techniques. The ds‐DNA/PAMAM/RGO/PGE was used as a working electrode to study the interaction between the RTX and salmon sperm ds‐DNA by DPV technique. Because of the interaction between the drug and DNA leads to a decrease in the guanine oxidation peak current, it was used as an indicator for the determination of the RTX. Under the optimized experimental conditions, a wide linear relationship between RTX concentration and guanine signal was obtained within the range of 7.0 to 60.0 μmol L⁻¹ and 60.0 to 300.0 μmol L⁻¹ with a low detection limit (0.56 μmol L⁻¹). To clarify the interaction mechanism between the RTX and the ds‐DNA, DPV and UV‐Vis measurements were used. The reproducibility, stability, and performance of the constructed biosensor was examined by quantitative measuring RTX in pharmaceutical and human serum samples with good precision (RSD; 2.0–6.0 %) and acceptable recoveries (100.04–101.95 %).

中文翻译：

DNA生物传感器修饰的PAMAM树状大分子/还原氧化石墨烯纳米复合材料对抗癌药Rituxan的定量生物检测

PAMAM树枝状大分子/还原氧化石墨烯纳米复合改性铅笔石墨电极（PAMAM / RGO / PGE）首次用于制造电化学DNA生物传感器，用于测定低浓度的Rituxan（RTX）。用FE‐SEM，EIS和CV技术对制成的生物传感器进行了表征。ds‐DNA / PAMAM / RGO / PGE被用作工作电极，通过DPV技术研究RTX与鲑鱼精子ds‐DNA之间的相互作用。由于药物和DNA之间的相互作用导致鸟嘌呤氧化峰电流的降低，因此它被用作确定RTX的指标。在优化的实验条件下，在7.0至60.0μmolL ^-1的范围内获得了RTX浓度与鸟嘌呤信号之间的宽线性关系。和60.0至300.0微摩尔大号^-1具有低检测限（0.56微摩尔大号^-1）。为了阐明RTX与ds‐DNA之间的相互作用机制，使用了DPV和UV‐Vis测量。通过定量测定药物和人血清样品中的RTX，以良好的精密度（RSD; 2.0–6.0％）和可接受的回收率（100.04–101.95％），检查了构建的生物传感器的重现性，稳定性和性能。

更新日期：2018-03-23

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