Gold Nanoparticle‐labeled Electrochemical Immunoassay Using Open Circuit Potential for Human Chorionic Gonadotropin Detection,Electroanalysis

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Gold Nanoparticle‐labeled Electrochemical Immunoassay Using Open Circuit Potential for Human Chorionic Gonadotropin Detection
Electroanalysis ( IF 2.7 ) Pub Date : 2018-04-14 , DOI: 10.1002/elan.201700797
Kanokwan Charoenkitamorn _{1,

2} , Phan Trong Tue ₁ , Miyuki Chikae ₁ , Orawon Chailapakul _{2,

3} , Yuzuru Takamura ₁

Affiliation

This study presents a new approach for an electrochemical immunoassay using gold nanoparticle (AuNP)‐labeled antibodies and pre‐oxidation and reduction processes, followed by open circuit potential (OCP) measurement. Detection of the pregnancy marker, human chorionic gonadotropin hormone (hCG), was used as a model. After preparation of a sandwich‐type immunosystem, the pre‐oxidation and reduction processes were applied, followed by OCP detection. The applied potential and time period were studied for the optimization of pre‐oxidation and reduction processes. We observed that the pre‐oxidation potential of 1.2 V for 60 s and reduction potential of −0.2 V for 30 s provided the highest OCP signal. The detection limit was 79 pg/mL using the optimal conditions. This system could be applied to a simplified and miniaturized diagnostic system for integration in compact analytical devices.

中文翻译：

使用开路电位的金纳米颗粒标记的电化学免疫法检测人绒毛膜促性腺激素

这项研究为使用金纳米颗粒（AuNP）标记的抗体，预氧化和还原过程以及开路电势（OCP）测量的电化学免疫测定提供了一种新方法。妊娠标记物，人绒毛膜促性腺激素（hCG）的检测被用作模型。准备好三明治型免疫系统后，进行预氧化和还原过程，然后进行OCP检测。研究了施加的电势和时间段，以优化预氧化和还原过程。我们观察到60 s时1.2 V的预氧化电位和30 s时-0.2 V的还原电位提供了最高的OCP信号。在最佳条件下，检出限为79 pg / mL。

更新日期：2018-04-14

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