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Electro‐oxidized Monolayer CVD Graphene Film Transducer for Ultrasensitive Impedimetric DNA Biosensor
Electroanalysis ( IF 2.7 ) Pub Date : 2018-04-16 , DOI: 10.1002/elan.201700654 Ali Benvidi 1, 2 , Nuvia M. Saucedo 1, 3 , Pankaj Ramnani 1 , Claudia Villarreal 4 , Ashok Mulchandani 1, 4 , Marzieh Dehghan Tezerjani 2 , Shahriar Jahanbani 2
Electroanalysis ( IF 2.7 ) Pub Date : 2018-04-16 , DOI: 10.1002/elan.201700654 Ali Benvidi 1, 2 , Nuvia M. Saucedo 1, 3 , Pankaj Ramnani 1 , Claudia Villarreal 4 , Ashok Mulchandani 1, 4 , Marzieh Dehghan Tezerjani 2 , Shahriar Jahanbani 2
Affiliation
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We report the effect of electrochemical anodization on the properties of monolayer graphene as the main aim of this research and consequently using the resulting label‐free impedimetric biosensor for DNA sequences detection. Monolayer graphene was grown by chemical vapor deposition (CVD) with methane as precursor on copper foil, transferred onto a glassy carbon electrode and electrochemically anodized. Raman spectroscopy and X‐Ray photo electron spectroscopy revealed enhancement of defect density, roughness and formation of C−O−C, C−O−H and C=O functional groups after anodization. Amine‐terminated poly T probe was linked covalently to the carboxylic groups of anodized graphene by the zero‐length linker to fabricate the impedance‐based DNA biosensor. The anodized graphene electrode demonstrated a superior performance for electrochemical impedance detection of DNA. The DNA biosensor showed a large linear dynamic range from 2.0×10−18 to 1.0×10−12 M with a limit of detection of 1.0×10−18 M using electrochemical impedance spectroscopy (EIS) method. Equivalent circuit modeling shows that DNA hybridization is detected through a change in charge transfer resistance.
中文翻译：
用于超灵敏阻抗DNA生物传感器的电氧化单层CVD石墨烯薄膜传感器
我们报告了电化学阳极氧化对单层石墨烯性能的影响，这是本研究的主要目的，因此，使用所得的无标记阻抗生物传感器进行DNA序列检测。通过化学气相沉积（CVD），以甲烷为前驱体在铜箔上生长单层石墨烯，将其转移到玻璃碳电极上并进行电化学阳极氧化。拉曼光谱和X射线光电子能谱显示，阳极氧化后缺陷密度，粗糙度和C-OC，C-OH和C = O官能团的形成得到增强。末端为末端的聚T探针通过零长度接头与阳极氧化石墨烯的羧基共价连接，从而构建了基于阻抗的DNA生物传感器。阳极氧化石墨烯电极表现出优异的DNA电化学阻抗检测性能。DNA生物传感器显示出2.0×10的大线性动态范围-18至1.0×10 -12 M， 使用电化学阻抗谱（EIS）方法的检出限为1.0×10 -18M。等效电路建模表明，通过电荷转移电阻的变化可以检测到DNA杂交。
更新日期：2018-04-16
中文翻译：
用于超灵敏阻抗DNA生物传感器的电氧化单层CVD石墨烯薄膜传感器
我们报告了电化学阳极氧化对单层石墨烯性能的影响，这是本研究的主要目的，因此，使用所得的无标记阻抗生物传感器进行DNA序列检测。通过化学气相沉积（CVD），以甲烷为前驱体在铜箔上生长单层石墨烯，将其转移到玻璃碳电极上并进行电化学阳极氧化。拉曼光谱和X射线光电子能谱显示，阳极氧化后缺陷密度，粗糙度和C-OC，C-OH和C = O官能团的形成得到增强。末端为末端的聚T探针通过零长度接头与阳极氧化石墨烯的羧基共价连接，从而构建了基于阻抗的DNA生物传感器。阳极氧化石墨烯电极表现出优异的DNA电化学阻抗检测性能。DNA生物传感器显示出2.0×10的大线性动态范围-18至1.0×10 -12 M， 使用电化学阻抗谱（EIS）方法的检出限为1.0×10 -18M。等效电路建模表明，通过电荷转移电阻的变化可以检测到DNA杂交。
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