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Fabrication of Miniaturised Screen-Printed Glucose Biosensors, using a Water-Based Ink, and the Evaluation of their Electrochemical Behaviour
Electroanalysis ( IF 2.7 ) Pub Date : 2018-04-25 , DOI: 10.1002/elan.201800104 G. Hughes 1 , R. M. Pemberton 1 , P. Nicholas 2 , J. P. Hart 1
Electroanalysis ( IF 2.7 ) Pub Date : 2018-04-25 , DOI: 10.1002/elan.201800104 G. Hughes 1 , R. M. Pemberton 1 , P. Nicholas 2 , J. P. Hart 1
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This paper describes a simple, convenient approach to the fabrication of microband electrodes and microband biosensors based on screen printing technology. These devices were printed in a three-electrode configuration on one strip; a silver/silver chloride electrode and carbon counter electrode served as reference and counter electrodes respectively. The working electrodes were fabricated by screen-printing a water-based carbon ink containing cobalt phthalocyanine for hydrogen peroxide detection. These were converted into a glucose microband biosensor by the addition of glucose oxidase into the carbon ink. In this paper, we discuss the fabrication and application of glucose microband electrodes for the determination of glucose in cell media. The dimensions (100 – 400 microns) of the microband electrodes result in radial diffusion, which results in steady state responses in the absence of stirring. The microband biosensors were investigated in cell media containing different concentrations of glucose using chronoamperometry. The device shows linearity for glucose determination in the range 0.5 mM to 2.5 mM in cell media. The screen-printed microband biosensor design holds promise as a generic platform for future applications in cell toxicity studies.
中文翻译：
使用水性墨水制造微型丝网印刷葡萄糖生物传感器，并评估其电化学行为
本文介绍了一种基于丝网印刷技术制造微带电极和微带生物传感器的简单、方便的方法。这些设备以三电极配置印刷在一个条带上；银/氯化银电极和碳对电极分别用作参比电极和对电极。工作电极是通过丝网印刷含有钴酞菁的水性碳墨来制造的，用于过氧化氢检测。通过将葡萄糖氧化酶添加到碳墨水中，将它们转化为葡萄糖微带生物传感器。在本文中，我们讨论了用于测定细胞培养基中葡萄糖的葡萄糖微带电极的制造和应用。微带电极的尺寸（100 – 400 微米）导致径向扩散，这会在没有搅拌的情况下产生稳态响应。使用计时电流法在含有不同浓度葡萄糖的细胞培养基中研究了微带生物传感器。该设备显示了细胞培养基中 0.5 mM 至 2.5 mM 范围内葡萄糖测定的线性。丝网印刷的微带生物传感器设计有望成为未来细胞毒性研究应用的通用平台。
更新日期：2018-04-25
中文翻译：
使用水性墨水制造微型丝网印刷葡萄糖生物传感器，并评估其电化学行为
本文介绍了一种基于丝网印刷技术制造微带电极和微带生物传感器的简单、方便的方法。这些设备以三电极配置印刷在一个条带上；银/氯化银电极和碳对电极分别用作参比电极和对电极。工作电极是通过丝网印刷含有钴酞菁的水性碳墨来制造的，用于过氧化氢检测。通过将葡萄糖氧化酶添加到碳墨水中，将它们转化为葡萄糖微带生物传感器。在本文中，我们讨论了用于测定细胞培养基中葡萄糖的葡萄糖微带电极的制造和应用。微带电极的尺寸（100 – 400 微米）导致径向扩散，这会在没有搅拌的情况下产生稳态响应。使用计时电流法在含有不同浓度葡萄糖的细胞培养基中研究了微带生物传感器。该设备显示了细胞培养基中 0.5 mM 至 2.5 mM 范围内葡萄糖测定的线性。丝网印刷的微带生物传感器设计有望成为未来细胞毒性研究应用的通用平台。
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