Schistosomiasis is a widespread chronic neglected tropical disease prevalent mostly in children in under-resourced rural areas. Its pathological effects have been clinically characterized, yet the molecular-level effects are understudied. In this study, the biochemical effects of Schistosoma mansoni infection and praziquantel treatment were studied in 130 preschool aged and 159 school aged infected children and 11 noninfected children in Azaguié, Côte d’Ivoire. Urine samples were collected prior to receiving 20, 40, or 60 mg/kg of praziquantel or a placebo, as well as 24 h post-treatment, and at the 3-week follow up. Urinary metabolic phenotypes were measured using ¹H NMR spectroscopy, and metabolic variation associated with S. mansoni infection and praziquantel administration was identified using multivariate statistical techniques. Discriminatory metabolic signatures were detected between heavily infected and noninfected children at baseline as well as according to the dose of praziquantel administered 24 h post treatment. These signatures were primarily associated with the metabolic activity of the gut microbiota, gut health and growth biomarkers and energy and liver metabolism. These analyses provide insights into the metabolic phenotype of schistosomiasis and treatment with praziquantel in two important demographics.

中文翻译：

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学龄前和学龄儿童对曼氏血吸虫感染的生化反应特征及吡喹酮治疗

血吸虫病是一种广泛的慢性被忽视的热带病，主要发生在资源贫乏的农村地区的儿童中。其病理作用已在临床上进行了表征，但分子水平的作用仍未得到研究。在这项研究中，在科特迪瓦的阿萨吉埃市的130名学龄前儿童和159名学龄儿童中的11名未感染儿童中研究了曼氏血吸虫感染和吡喹酮治疗的生化作用。在接受20、40或60 mg / kg吡喹酮或安慰剂之前以及治疗后24小时以及3周的随访中收集尿液样本。使用¹ H NMR光谱测量尿液代谢表型，以及与曼氏链球菌相关的代谢变化使用多元统计技术确定感染和吡喹酮给药。在基线以及根据治疗后24小时服用吡喹酮的剂量，在重度感染和未感染的儿童之间检测到歧视性代谢特征。这些特征主要与肠道菌群的代谢活性，肠道健康和生长生物标志物以及能量和肝脏代谢有关。这些分析为两个重要的人口统计学领域的血吸虫病代谢表型和吡喹酮治疗提供了见识。