Application of HPLC-DAD and spectrophotometric continuous wavelet transform methods for simultaneous determination of amoxicillin and diclofenac in their pure and capsule dosage forms,Analytical Methods

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Application of HPLC-DAD and spectrophotometric continuous wavelet transform methods for simultaneous determination of amoxicillin and diclofenac in their pure and capsule dosage forms
Analytical Methods ( IF 2.7 ) Pub Date : 2018-04-27 00:00:00 , DOI: 10.1039/c8ay00621k
Fathy M. Salama _{1,

2,

3,

4,

5} , Khalid A. Attia _{1,

2,

3,

4,

5} , Ahmed A. Abouserie _{1,

2,

3,

4,

5} , Ahmed El-Olemy _{1,

2,

3,

4,

5} , Ebrahim Abolmagd _{1,

2,

3,

4,

5}

Affiliation

Two methods have been developed and validated for simultaneous determination of amoxicillin sodium and diclofenac sodium in their pure and combined pharmaceutical dosage forms. The first method was HPLC-DAD; the chromatographic separation and resolution has been carried out using a reversed phase BDS Hypersil C18 column with a mobile phase consisting of methanol : acetonitrile : water : orthophosphoric acid (60 : 30 : 9 : 1 by volume), at a flow rate of 1 mL minute⁻¹ and UV detection at 250 nm. The retention times were found to be 3.906 and 6.997 minutes for amoxicillin and diclofenac respectively. The second method was continuous wavelet transform (CWT), which is based on derivative calculation of spectrophotometric spectral data of both drugs in their binary mixture, and the zero crossing point for amoxicillin and diclofenac was found to be at 233 and 247 nm, respectively. The results obtained were statistically compared to reference methods and there were no significant differences between the proposed methods and the reference methods regarding the accuracy and precision. The method was validated according to ICH guidelines and the results were satisfactory.

中文翻译：

HPLC-DAD和分光光度连续小波变换方法同时测定阿莫西林和双氯芬酸的纯和胶囊剂型

已开发并验证了两种方法，可同时测定纯和组合药物剂型中的阿莫西林钠和双氯芬酸钠。第一种方法是HPLC-DAD。使用反相BDS Hypersil C18色谱柱进行色谱分离和分离，流动相由甲醇：乙腈：水：正磷酸（体积比为60：30：9：1）组成，流速为1 mL分钟^-1和在250 nm处进行紫外线检测。阿莫西林和双氯芬酸的保留时间分别为3.906分钟和6.997分钟。第二种方法是连续小波变换（CWT），该方法基于两种药物在其二元混合物中的分光光度光谱数据的导数计算，并且阿莫西林和双氯芬酸的零交叉点分别位于233 nm和247 nm。将获得的结果与参考方法进行统计比较，并且在准确性和精密度方面，建议的方法与参考方法之间没有显着差异。该方法根据ICH指南进行了验证，结果令人满意。

更新日期：2018-04-27

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