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Organized Arrangement of Calcium Carbonate Crystals, Directed by a Rationally Designed Protein
Crystal Growth & Design ( IF 3.2 ) Pub Date : 2018-04-25 00:00:00 , DOI: 10.1021/acs.cgd.8b00365 Hang Ping , Yamin Wan , Hao Xie , Jingjing Xie , Weimin Wang , Hao Wang , Zuhair A. Munir 1 , Zhengyi Fu
Crystal Growth & Design ( IF 3.2 ) Pub Date : 2018-04-25 00:00:00 , DOI: 10.1021/acs.cgd.8b00365 Hang Ping , Yamin Wan , Hao Xie , Jingjing Xie , Weimin Wang , Hao Wang , Zuhair A. Munir 1 , Zhengyi Fu
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The integration of a “brick and mortar” structure and superior fracture toughness of nacre has attracted intensive attention recently. Elucidating the interactions between biomacromolecules and inorganic phases is beneficial to understand the structure-forming process of nacre. Herein, a recombinant protein, ChiSifiCa, mimicking features of the organic matrix in nacre is rationally designed. This protein contains three functional domains, chitin binding (Chi), silk fibroin (Sifi), and calcium ion binding (Ca). The domain order in ChiSifiCa is in the same manner as the distribution of organic matrix in nacre. When ChiSifiCa binds to the surface of chitin, it provides a confined microenvironment facilitating CaCO3 mineralization. The formation of spherical vaterite minerals with a hollow structure was observed under the function of ChiSifiCa. These minerals are assembled by well-aligned nanoplatelets and nanoparticles, distributed in the outer and inner region of the sphere, respectively. The nanoparticles were oriented radially from the center to the edge. These nanoplatelets with stacked multilayers display almost the same crystallographic orientation. During the biomineralization process, there is change of secondary structure of ChiSifiCa from random coil to α-helix.
中文翻译：
由合理设计的蛋白质指导的碳酸钙晶体的有组织排列
近来，“砖和灰浆”结构的整合以及珍珠母的优异断裂韧性引起了广泛的关注。阐明生物大分子与无机相之间的相互作用有助于理解珍珠母的结构形成过程。本文中，合理设计了模仿珍珠母中有机基质特征的重组蛋白ChiSifiCa。该蛋白包含三个功能域，几丁质结合（Chi），丝素蛋白（Sifi）和钙离子结合（Ca）。ChiSifiCa中的域顺序与珍珠母中有机基质的分布方式相同。当ChiSifiCa结合到几丁质表面时，它提供了一个封闭的微环境，促进了CaCO 3的生成。矿化。在ChiSifiCa的作用下，观察到了具有空心结构的球形球ate石矿物的形成。这些矿物由排列良好的纳米片和纳米颗粒组装而成，分别分布在球体的外部和内部。纳米颗粒从中心到边缘径向取向。这些具有多层堆叠的纳米片表现出几乎相同的晶体学取向。在生物矿化过程中，ChiSifiCa的二级结构从无规卷曲变为α-螺旋。
更新日期：2018-04-25
中文翻译：
由合理设计的蛋白质指导的碳酸钙晶体的有组织排列
近来，“砖和灰浆”结构的整合以及珍珠母的优异断裂韧性引起了广泛的关注。阐明生物大分子与无机相之间的相互作用有助于理解珍珠母的结构形成过程。本文中，合理设计了模仿珍珠母中有机基质特征的重组蛋白ChiSifiCa。该蛋白包含三个功能域，几丁质结合（Chi），丝素蛋白（Sifi）和钙离子结合（Ca）。ChiSifiCa中的域顺序与珍珠母中有机基质的分布方式相同。当ChiSifiCa结合到几丁质表面时，它提供了一个封闭的微环境，促进了CaCO 3的生成。矿化。在ChiSifiCa的作用下，观察到了具有空心结构的球形球ate石矿物的形成。这些矿物由排列良好的纳米片和纳米颗粒组装而成，分别分布在球体的外部和内部。纳米颗粒从中心到边缘径向取向。这些具有多层堆叠的纳米片表现出几乎相同的晶体学取向。在生物矿化过程中，ChiSifiCa的二级结构从无规卷曲变为α-螺旋。
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