This study aimed to investigate the mycoparasitism of Botrytis cinerea, the pathogen of scape and umbel blights of onion seed crops, by endophytic Talaromyces pinophilus. The dual culture test showed that the antagonistic potentiality of T. pinophilus against B. cinerea depend on the mycoparasitism that was morphologically detected by the formation of mycelial overgrowth. Moreover, the light micrograph of the mycelia at the contact zone exhibited that the hyphae of T. pinophilus penetrated and grew intracellularly inside the hyphae of B. cinerea. A more illustrative figure of the establishment of coiled hyphae as well as the conformation of the penetration process was assayed by SEM and TEM analyses. SEM micrograph revealed that the hyphae of T. pinophilus grew along hyphae of B. cinerea, attached, coiled around the host hypha and generated pseudoappressorium. A clear disintegration of cell wall of the host hypha was observed at the penetration site. The micrographs of TEM exhibited the ability of T. pinophilus to produce pseudoappressorium, penetrate and then entere a hypha of B. cinerea causing distinct cytoplasmic disorganization. High activities of cell wall degrading enzymes (chitinase, lipase and protease) involved in the mycoparasitism were evaluated by the endophytic T. pinophilus. In conclusion, this study demonstrated that the endophytic T. pinophilus may be a promising biocontrol agent against phytopathogenic fungi instead of chemical fungicides.

这项研究的目的是通过内生的嗜睡松毛虫（Talaromyces pinophilus）研究灰葡萄孢（Botrytis cinerea）的真菌副寄生虫病。双重培养试验表明，嗜热支原体对灰质芽孢杆菌的拮抗潜力取决于通过菌丝体过度生长的形成在形态学上检测到的支原体分枝。此外，在接触区的菌丝体的光学显微照片显示，嗜血链球菌的菌丝在灰葡萄孢菌的菌丝内部渗透并在细胞内生长。通过SEM和TEM分析测定了卷曲菌丝的建立以及穿透过程的构象的更说明性的图。扫描电镜显微照片显示，嗜热链球菌的菌丝沿着灰质芽孢杆菌的菌丝生长，附着，缠绕在宿主菌丝周围，并产生假顶叶。在穿透位点观察到宿主菌丝的细胞壁明显崩解。透射电镜的显微照片显示，嗜油性支原体产生假顶突，穿透并进入灰质芽孢杆菌的菌丝的能力。引起明显的细胞质混乱。通过内生T. pinophilus评估了支原体炎涉及的细胞壁降解酶（几丁质酶，脂肪酶和蛋白酶）的高活性。总之，这项研究表明，内生的嗜热性嗜血杆菌可能是一种有前途的防治植物病原真菌而不是化学杀真菌剂的生物防治剂。