We designed and constructed a novel aptasensor based on the porous nanostructured bimetallic NiFe-oxides embedded with the mesoporous carbon (represented by NiO_xFeO_y@mC) for sensitively detecting adenosine triphosphate (ATP), of which the porous NiO_xFeO_y@mC was derived from the hollow NiFe Prussian blue analogue (hollow NiFe PBA) by calcinating under high temperature. Owning to the excellent electrochemical activity originated from the metal oxides and mesoporous carbon and the strong binding interaction between the aptamer strands and the nanostructure hybrid, the formed porous NiO_xFeO_y@mC composite calcinated at 900 °C exhibited superior sensitivity toward ATP determination in comparison with other porous nanocubes obtained at 500 and 700 °C. The proposed aptasensor not only revealed a wide linear range from 5.0 fg·mL⁻¹ to 5.0 ng·mL⁻¹ with a extremely low detection limit of 0.98 fg·mL⁻¹ (1.62 fM) (S/N=3), but also displayed high selectivity towards other interferences, good stability and reproducibility, and acceptable applicability. Therefore, this proposed approach provides a promising platform for ultra-sensitive detection of ATP, further having the potential applications on diagnosis of ATP-related diseases.

我们设计并构建了一种新型的适体传感器，该传感器基于嵌入中孔碳的多孔纳米结构双金属NiFe-氧化物（以NiO _x FeO _y @mC表示），用于灵敏地检测三磷酸腺苷（ATP），其中多孔NiO _x FeO _y @mC通过在高温下煅烧而得自空心NiFe普鲁士蓝类似物（空心NiFe PBA）。由于金属氧化物和介孔碳具有出色的电化学活性以及适体链与纳米结构杂化物之间的强结合相互作用，所形成的多孔NiO _x FeO _y与在500和700°C下获得的其他多孔纳米立方体相比，在900°C下煅烧的@mC复合材料对ATP测定具有更高的灵敏度。所提出的适体传感器不仅显示了从5.0 fg·mL ^-1到5.0 ng·mL ^-1的宽线性范围，而且检测限极低，仅为0.98 fg·mL ^-1（1.62 fM）（S / N = 3），还显示出对其他干扰物的高选择性，良好的稳定性和可重复性以及可接受的适用性。因此，该提出的方法为ATP的超灵敏检测提供了有希望的平台，进一步在诊断与ATP有关的疾病中具有潜在的应用。