A novel concept is proposed in which alginate capsules containing a model probiotic Lactobacillus plantarum strain are coated with different surfactants with the aim to enhance cell survival during passage initially through simulated gastric (SGF) and then intestinal (SIF) fluid. The surfactants investigated included the anionic sodium dodecyl sulphate (SDS) and ammonium lauryl sulphate (ALS), the cationic dimethyldioctadecylammonium chloride (DDAC), benzalkonium chloride (BZK) and hexadecyltrimethylammonium bromide (CTAB), and the zwitterionic lecithin. Coating the alginate capsules with CTAB, BZK, ALS and SDS resulted in worst survival (∼ 4-9 log CFU/g decrease) compared to uncoated capsules (∼3 log CFU/g decrease), after 1 hour exposure to SGF and two hours in SIF, which was most likely associated with their gradual penetration inside the microcapsules, as shown by confocal microscopy, and their antimicrobial effects. Coating the alginate capsules with DDAC improved cell survival compared to uncoated capsules (∼1.2 CFU/g decrease), whereas coating with lecithin improved cell survival considerably, resulting in almost complete recovery of viable cells in SGF and SIF (∼ 0.3 log CFU/g decrease). Although the interaction between alginate and lecithin was relatively weak as demonstrated by turbidity and contact angle measurements, it is likely that the protection was associated with the fact that lecithin was able to penetrate into the capsule rapidly, an observation that was supported by the fact that lecithin enhanced the viability of free cells in SGF and SIF. Lecithin has significant potential of being used as a coating material for probiotic containing capsules.

提出了一种新概念，其中藻酸盐胶囊包含模型益生菌植物乳杆菌该菌株用不同的表面活性剂包被，目的是在最初通过模拟胃液（SGF）然后通过肠液（SIF）的过程中提高细胞存活率。研究的表面活性剂包括阴离子十二烷基硫酸钠（SDS）和十二烷基硫酸铵（ALS），阳离子二甲基二十八烷基氯化铵（DDAC），苯扎氯铵（BZK）和十六烷基三甲基溴化铵（CTAB）以及两性离子卵磷脂。在暴露于SGF 1小时和2小时后，与未包衣胶囊相比，用CTAB，BZK，ALS和SDS包被海藻酸盐胶囊导致最差的生存（降低约4-9 log CFU / g）（降低约3 log CFU / g）。共聚焦显微镜显示，SIF中最有可能与它们逐渐渗透到微囊内有关，以及它们的抗微生物作用。与未包衣胶囊相比，用DDAC包被藻酸盐胶囊可提高细胞存活率（降低约1.2 CFU / g），而卵磷脂包衣可显着提高细胞存活率，从而使SGF和SIF中的活细胞几乎完全恢复（约0.3 log CFU / g）。减少）。尽管通过浊度和接触角测量表明藻酸盐和卵磷脂之间的相互作用相对较弱，但保护作用可能与卵磷脂能够迅速渗透到胶囊中这一事实有关，这一观察结果得到了以下事实的支持：卵磷脂增强了SGF和SIF中游离细胞的活力。卵磷脂具有用作含益生菌胶囊的包衣材料的巨大潜力。而用卵磷脂包被可显着提高细胞存活率，从而使SGF和SIF中的活细胞几乎完全恢复（减少约0.3 log CFU / g）。尽管通过浊度和接触角测量表明藻酸盐和卵磷脂之间的相互作用相对较弱，但保护作用可能与卵磷脂能够迅速渗透到胶囊中这一事实有关，这一观察结果得到了以下事实的支持：卵磷脂增强了SGF和SIF中游离细胞的活力。卵磷脂具有用作含益生菌胶囊的包衣材料的巨大潜力。而用卵磷脂包被可显着提高细胞存活率，从而使SGF和SIF中的活细胞几乎完全恢复（减少约0.3 log CFU / g）。尽管通过浊度和接触角测量表明藻酸盐和卵磷脂之间的相互作用相对较弱，但保护作用可能与卵磷脂能够迅速渗透到胶囊中这一事实有关，这一观察结果得到了以下事实的支持：卵磷脂增强了SGF和SIF中游离细胞的活力。卵磷脂具有用作含益生菌胶囊的包衣材料的巨大潜力。尽管通过浊度和接触角测量表明藻酸盐和卵磷脂之间的相互作用相对较弱，但保护作用可能与卵磷脂能够迅速渗透到胶囊中这一事实有关，这一观察结果得到了以下事实的支持：卵磷脂增强了SGF和SIF中游离细胞的活力。卵磷脂具有用作含益生菌胶囊的包衣材料的巨大潜力。尽管通过浊度和接触角测量表明藻酸盐和卵磷脂之间的相互作用相对较弱，但保护作用可能与卵磷脂能够迅速渗透到胶囊中这一事实有关，这一观察结果得到了以下事实的支持：卵磷脂增强了SGF和SIF中游离细胞的活力。卵磷脂具有用作含益生菌胶囊的包衣材料的巨大潜力。