Mass spectrometry (MS)-based methods for analyzing protein higher order structures have gained increasing application in the field of biopharmaceutical development. The predominant methods used in this area include native MS, hydrogen deuterium exchange-MS, covalent labeling, cross-linking and limited proteolysis. These MS-based methods will be briefly described in this article, followed by a discussion on how these methods contribute at different stages of discovery and development of protein therapeutics.

中文翻译：

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基于MS的重组蛋白在生物药物设计，优化和开发中的构象分析

基于质谱（MS）的分析蛋白质高级结构的方法在生物药物开发领域中的应用越来越广泛。该领域使用的主要方法包括天然MS，氢氘交换MS，共价标记，交联和有限的蛋白水解。这些基于MS的方法将在本文中进行简要描述，然后讨论这些方法如何在蛋白质治疗剂的发现和开发的不同阶段做出贡献。