Spatio-temporal organization of the cell nucleus adapts to and regulates genomic processes. Microscopy approaches that enable direct monitoring of specific chromatin sites in single cells and in real time are needed to better understand the dynamics involved. In this chapter, we describe the principle and development of ANCHOR, a novel tool for DNA labelling in eukaryotic cells. Protocols for use of ANCHOR to visualize a single genomic locus in eukaryotic cells are presented. We describe an approach for live cell imaging of a DNA locus during the entire cell cycle in human breast cancer cells.

中文翻译：

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使用ANCHOR DNA标记系统对真核细胞中特定基因组位点进行实时成像

细胞核的时空组织适应并调节基因组过程。为了更好地了解所涉及的动力学，需要能够直接和实时地直接监测单个细胞中特定染色质位点的显微镜方法。在本章中，我们描述了ANCHOR的原理和发展，ANCHOR是一种在真核细胞中进行DNA标记的新型工具。提出了使用ANCHOR可视化真核细胞中单个基因组基因座的方案。我们描述了一种在人类乳腺癌细胞的整个细胞周期中对DNA基因座进行活细胞成像的方法。