Introducing synthetic constructs into bacteria often carries a burden that leads to reduced fitness and selective pressure for organisms to mutate their constructs and hence to a reduced functional lifetime. Understanding burden requires suitable methods for accurate measurement and quantification. We develop a dynamic growth model from physiologically relevant first-principles that allows parameters relevant to burden to be extracted from standard growth curves. We test several possibilities for the response of a bacterium to a new environment in terms of resource allocation. We find that burden manifests in the time taken to respond to new conditions as well as the rate of growth in exponential phase. Furthermore, we see that the presence of a synthetic construct hastens the reduction of ribosomes when approaching stationary phase, altering memory effects from previous periods of growth.

中文翻译：

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响应综合构造的动态资源分配模型

将合成的构建体引入细菌通常会带来负担，从而导致有机体适应性降低和选择性压力，以使生物体对其构建体进行突变，从而缩短其功能寿命。了解负担需要采用正确的方法进行准确的测量和定量。我们从生理学相关的第一性原理开发了动态生长模型，该模型允许从标准生长曲线中提取与负担相关的参数。在资源分配方面，我们测试了细菌对新环境的反应的几种可能性。我们发现负担体现在应对新情况所需的时间以及指数阶段的增长率。此外，我们看到，当接近固定相时，合成构建体的存在会加速核糖体的减少，