An isothermal single base extension based lateral flow biosensor and electrochemical assay for gene point mutation detection†,Analytical Methods

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An isothermal single base extension based lateral flow biosensor and electrochemical assay for gene point mutation detection†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-04-24 00:00:00 , DOI: 10.1039/c8ay00746b
Ning Sun _{1,

2,

3} , Qi Guo _{3,

4,

5,

6,

7} , Xiaomei Li _{1,

2,

3} , Jitao Chen _{1,

2,

3} , Xiaowen Liu _{3,

4,

5,

6,

7} , Kwok-Yin Wong _{3,

8,

9,

10,

11} , Zhiyuang Fang _{1,

2,

3}

Affiliation

Gene point mutations are commonly associated with the development of cancer and other diseases. The rapid and accurate detection of mutation is increasingly needed in personalized therapeutic regimes. In this proof-of-concept study, an isothermal single base extension (SBE) reaction was developed for the simple and fast amplification of mutations. Combined with a lateral flow biosensor and electrochemical assay, a limit of detection of 1 nM and 1 pM were achieved, respectively. Compared with traditional PCR-based assays, this approach is faster, simpler and more cost-effective. Furthermore, the reduced experimental requirements enable its integration with portable micro-fluidic devices, forming a simple and automatic point of care device for point mutation detections.

中文翻译：

基于等温单碱基延伸的侧向流生物传感器和电化学测定法，用于基因点突变检测†

基因点突变通常与癌症和其他疾病的发展有关。在个性化治疗方案中，越来越需要快速，准确地检测突变。在此概念验证研究中，开发了等温单碱基延伸（SBE）反应，用于简单，快速地扩增突变。结合侧向流动生物传感器和电化学分析，分别达到了1 nM和1 pM的检测限。与传统的基于PCR的测定相比，该方法更快，更简单且更具成本效益。此外，减少的实验要求使其能够与便携式微流控设备集成，从而形成用于点突变检测的简单且自动的即时护理设备。

更新日期：2018-04-24

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