A novel competitive-displacement fluorescence assay for l-penicillamine based on the reaction between the target and N-acetyl-l-cysteine-capped CdTe quantum dots for copper ions†,Analytical Methods

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A novel competitive-displacement fluorescence assay for l-penicillamine based on the reaction between the target and N-acetyl-l-cysteine-capped CdTe quantum dots for copper ions†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-04-23 00:00:00 , DOI: 10.1039/c8ay00515j
Yunmei Huang _{1,

2,

3,

4} , Jidong Yang _{1,

2,

3,

4,

5} , Haiyan Yuan _{1,

2,

3,

4} , Yuan Guo _{1,

2,

3,

4} , Xiaoqing Zeng _{1,

2,

3,

4} , Jiawei Cheng _{1,

2,

3,

4} , Yuhui Zhang _{1,

2,

3,

4}

Affiliation

D-Penicillamine (D-PA), as the dominant enantiomer, is usually used in many clinical drugs, but the other enantiomer, L-penicillamine (L-PA) is a toxic enantiomer. Therefore, it is of great significance to analyze and detect L-PA in biomedical or environmental samples. CdTe quantum dots (CdTe QDs) were modified by N-acetyl-L-cysteine (NALC) as novel fluorescent probes and were successfully utilized for the determination of L-penicillamine. The fluorescence of the CdTe QDs decreased significantly in the presence of cupric ions (Cu²⁺) due to the binding of Cu²⁺ to NALC on the surface of the CdTe QDs, but the addition of L-PA immediately resulted in efficient fluorescence recovery of the modulated CdTe QDs. The experimental results showed that the pH of the buffer solution and the concentration of Cu²⁺ affected the fluorescence intensity upon adding L-PA. Under the optimum conditions, the recovered fluorescence intensity was linearly proportional to the increasing L-PA concentration in the range of 2 × 10⁻⁷ to 4 × 10⁻⁶ mol L⁻¹, with correlation coefficient R² = 0.9980. So, a new, simple and rapid method for the determination of L-PA has been developed and its potential has been demonstrated in human serum samples and environmental samples with satisfactory results.

中文翻译：

提供一种新颖的有竞争力-位移荧光测定升青霉胺基于目标之间的反应Ñ乙酰基升-半胱氨酸-封端的对铜离子的CdTe量子点†

作为主要的对映异构体，D-青霉胺（D -PA）通常用于许多临床药物中，但其他对映异构体L-青霉胺（L -PA）是有毒的对映异构体。因此，分析和检测生物医学或环境样品中的L -PA具有重要意义。N-乙酰基-L-半胱氨酸（NALC）修饰了CdTe量子点（CdTe QDs）作为新型荧光探针，并成功地用于测定L-青霉胺。在CdTe量子点的荧光在二价铜离子（Cu的存在显著降低²⁺）由于Cu的结合²⁺在CdTe QDs的表面上用NALC标记，但添加L -PA立即导致调制的CdTe QDs的有效荧光恢复。实验结果表明，加入L -PA后，缓冲溶液的pH值和Cu ²⁺的浓度会影响荧光强度。在最佳条件下，恢复的荧光强度与L -PA浓度在2×10 ^-7至4×10 ^-6 mol L ^-1范围内呈线性比例关系，相关系数R ² = 0.9980。因此，一种新的，简单而快速的测定L的方法已经开发了-PA，并且已经在人血清样品和环境样品中证明了其潜力，并获得了令人满意的结果。

更新日期：2018-04-23

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