Based on 98 public and internal small RNA high throughput sequencing libraries, we mapped small RNAs to the genome of the model organism Arabidopsis thaliana and defined loci based on their expression using an empirical Bayesian approach. The resulting loci were subsequently classified based on their genetic and epigenetic context as well as their expression properties. We present the results of this classification, which broadly conforms to previously reported divisions between transcriptional and post-transcriptional gene silencing small RNAs, and to PolIV and PolV dependencies. However, we are able to demonstrate the existence of further subdivisions in the small RNA population of functional significance. Moreover, we present a framework for similar analyses of small RNA populations in all species.

中文翻译：

---

朝向注释植物表观基因组：拟南芥小 RNA 基因座图谱。

基于 98 个公共和内部小 RNA 高通量测序文库，我们将小 RNA 映射到模式生物拟南芥的基因组，并使用经验贝叶斯方法根据它们的表达定义位点。随后根据它们的遗传和表观遗传背景以及它们的表达特性对产生的基因座进行分类。我们展示了这种分类的结果，它大致符合先前报道的转录和转录后基因沉默小 RNA 之间的划分，以及 PolIV 和 PolV 依赖性。然而，我们能够证明在具有功能意义的小 RNA 群体中存在进一步的细分。此外，我们提出了一个对所有物种中的小 RNA 种群进行类似分析的框架。