Based on 98 public and internal small RNA high throughput sequencing libraries, we mapped small RNAs to the genome of the model organism Arabidopsis thaliana and defined loci based on their expression using an empirical Bayesian approach. The resulting loci were subsequently classified based on their genetic and epigenetic context as well as their expression properties. We present the results of this classification, which broadly conforms to previously reported divisions between transcriptional and post-transcriptional gene silencing small RNAs, and to PolIV and PolV dependencies. However, we are able to demonstrate the existence of further subdivisions in the small RNA population of functional significance. Moreover, we present a framework for similar analyses of small RNA populations in all species.

中文翻译：

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注释植物表观基因组：拟南芥小 RNA 基因座图。


基于 98 个公共和内部小 RNA 高通量测序文库，我们将小 RNA 映射到模式生物拟南芥的基因组，并使用经验贝叶斯方法根据其表达定义基因座。随后根据所得基因座的遗传和表观遗传背景及其表达特性对其进行分类。我们提出了这种分类的结果，该结果大致符合先前报道的转录和转录后基因沉默小RNA之间的划分，以及PolIV和PolV依赖性。然而，我们能够证明具有功能意义的小RNA群体中存在进一步的细分。此外，我们提出了一个对所有物种的小 RNA 群体进行类似分析的框架。