Psoriasis is a common inflammatory disease. It affects 1-3% of the population worldwide and is associated with increasing medical costs every year. Typical psoriatic skin lesions are reddish, thick, scaly plaques that can occur on multiple skin sites all over the body. Topical application of imiquimod (IMQ), a toll-like receptor (TLR)7 agonist and potent immune system activator, can induce and exacerbate psoriasis. Previous studies have demonstrated that isoflavone extract has an antioxidant effect which may help decrease inflammation and inflammatory pain. Through in vivo studies in mice, we found that the topical application to the shaved back and right ear of mice of isoflavone extract prior to IMQ treatment significantly decreased trans-epidermal water loss (TEWL), erythema, blood flow speed, and ear thickness, while it increased surface skin hydration, and attenuated epidermal hyperplasia and inflammatory cell infiltration. Through in vitro experiments, we found that isoflavone extract can reduce IL-22, IL-17A, and TNF-α-induced MAPK, NF-κB, and JAK-STAT activation in normal human epidermal keratinocytes. At the mRNA level, we determined that isoflavone extract attenuated the increased response of the TNF-α-, IL-17A-, and IL-22- related pathways. These results indicate that isoflavone extract has great potential as an anti-psoriatic agent and in the treatment of other inflammatory skin diseases.

中文翻译：

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异黄酮提取物对牛皮癣的治疗潜力和分子机理。

牛皮癣是一种常见的炎症性疾病。它影响着全世界1-3％的人口，并且每年都在增加医疗费用。典型的银屑病皮肤病变为淡红色，厚鳞状斑块，可出现在全身的多个皮肤部位。咪喹莫特（IMQ）是一种收费型受体（TLR）7激动剂和有效的免疫系统激活剂，局部使用可引起并加剧牛皮癣。先前的研究表明，异黄酮提取物具有抗氧化作用，可以帮助减轻炎症和炎症性疼痛。通过对小鼠的体内研究，我们发现，在IMQ处理之前，将异黄酮提取物局部施用于小鼠的剃毛后背和右耳，可显着降低经表皮水分流失（TEWL），红斑，血流速度和耳朵厚度，同时增加表面皮肤的水合作用，并减轻表皮增生和炎性细胞浸润。通过体外实验，我们发现异黄酮提取物可以减少正常人表皮角质形成细胞中IL-22，IL-17A和TNF-α诱导的MAPK，NF-κB和JAK-STAT活化。在mRNA水平上，我们确定异黄酮提取物减弱了TNF-α-，IL-17A-和IL-22相关途径的应答增加。这些结果表明异黄酮提取物作为抗银屑病药物和治疗其他炎症性皮肤病具有巨大潜力。和正常人表皮角质形成细胞中的JAK-STAT激活。在mRNA水平上，我们确定异黄酮提取物减弱了TNF-α-，IL-17A-和IL-22相关途径的应答增加。这些结果表明异黄酮提取物作为抗银屑病药物和治疗其他炎症性皮肤病具有巨大潜力。和正常人表皮角质形成细胞中的JAK-STAT激活。在mRNA水平上，我们确定异黄酮提取物减弱了TNF-α-，IL-17A-和IL-22相关途径的应答增加。这些结果表明异黄酮提取物作为抗银屑病药物和治疗其他炎症性皮肤病具有巨大潜力。