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Anti-Cancer Effects of Emodin on HepG2 Cells as Revealed by 1H NMR Based Metabolic Profiling
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-04-26 , DOI: 10.1021/acs.jproteome.8b00029 Yue-Xiao Xing 1 , Ming-Hui Li 1 , Liang Tao 1 , Ling-Yu Ruan 1 , Wei Hong 1 , Cheng Chen 1 , Wen-Long Zhao 1 , Han Xu 1 , Jian-Feng Chen 1 , Jun-Song Wang 1
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-04-26 , DOI: 10.1021/acs.jproteome.8b00029 Yue-Xiao Xing 1 , Ming-Hui Li 1 , Liang Tao 1 , Ling-Yu Ruan 1 , Wei Hong 1 , Cheng Chen 1 , Wen-Long Zhao 1 , Han Xu 1 , Jian-Feng Chen 1 , Jun-Song Wang 1
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Hepatic carcinoma is one of the most common cancers in the world, with a high incidence. Emodin is an anthraquinone derived from Polygonum multiflorum Thunb, possessing anti-cancer activity. The purpose of this study is to investigate the anti-cancer effect of different dosages of emodin on HepG2 cells using a 1H NMR based metabolic approach complemented with qRT-PCR and flow cytometry to identify potential markers and discover the targets to explore the underlying mechanism. Emodin can dose-dependently inhibit the growth of HepG2 cells, perturb cell cycle progression, down-regulate the expression of genes and proteins related to glycolysis, and trigger intracellular ROS generation. Orthogonal signal correction partial least-squares discriminant analysis (OSC-PLS-DA) and correlation network analysis of the 1H NMR data showed significant changes in many endogenous metabolites after emodin exposure concerning oxidative stress and disturbances in amino acid and energy metabolism. These findings are helpful to understand the anti-cancer mechanism of emodin and provide a theoretical basis for its future application and development.
中文翻译：
大黄素对HepG2细胞的抗癌作用，通过基于1 H NMR的代谢谱分析揭示
肝癌是世界上最常见的癌症之一，发病率很高。大黄素是一种从何首乌中提取的蒽醌，具有抗癌活性。这项研究的目的是调查大黄素的不同剂量对使用HepG2细胞的抗癌作用1基于1 H NMR的代谢方法与qRT-PCR和流式细胞仪相辅相成，以识别潜在的标志物并发现靶标以探索潜在的机制。大黄素可以剂量依赖性地抑制HepG2细胞的生长，扰动细胞周期进程，下调与糖酵解相关的基因和蛋白质的表达，并触发细胞内ROS的产生。正交信号校正偏最小二乘判别分析（OSC-PLS-DA）和相关网络分析11 H NMR数据显示大黄素暴露后许多内源性代谢物的显着变化，涉及氧化应激以及氨基酸和能量代谢紊乱。这些发现有助于理解大黄素的抗癌机制，并为大黄素的未来应用和发展提供理论依据。
更新日期：2018-04-26
中文翻译：
大黄素对HepG2细胞的抗癌作用，通过基于1 H NMR的代谢谱分析揭示
肝癌是世界上最常见的癌症之一，发病率很高。大黄素是一种从何首乌中提取的蒽醌，具有抗癌活性。这项研究的目的是调查大黄素的不同剂量对使用HepG2细胞的抗癌作用1基于1 H NMR的代谢方法与qRT-PCR和流式细胞仪相辅相成，以识别潜在的标志物并发现靶标以探索潜在的机制。大黄素可以剂量依赖性地抑制HepG2细胞的生长，扰动细胞周期进程，下调与糖酵解相关的基因和蛋白质的表达，并触发细胞内ROS的产生。正交信号校正偏最小二乘判别分析（OSC-PLS-DA）和相关网络分析11 H NMR数据显示大黄素暴露后许多内源性代谢物的显着变化，涉及氧化应激以及氨基酸和能量代谢紊乱。这些发现有助于理解大黄素的抗癌机制，并为大黄素的未来应用和发展提供理论依据。
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