AbstractIncreasing sensitivity and decreasing assay time are two of the most crucial goals in the development of biosensing devices, such as immunoassays, protein electrophoresis, and bioimaging nanoprobes. We have developed three-dimensional (3D) nano/microstructured platforms for biosensing devices aimed at highly sensitive and rapid immunoassays. One such platform is a nanosphere structure with poly[2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-nitrophenyloxycarbonyl poly(ethylene glycol) methacrylate] (PMBN) prepared by the electrospray deposition (ESD) method. Compared with flat PMBN, the 3D nanosphere surface enables the immobilization of a large amount of antibody to enhance the specific signal of the immunoassay. Furthermore, PMBN is resistant to non-specific protein adsorption. Thus, this 3D nanosphere structure can be applied in microchip environments, leading to highly sensitive and rapid immunoassays. To obtain a more rapid assay that takes at most 5 min, we utilized another platform: a 3D polystyrene (PS) microfiber structure prepared by ESD paired with vacuum pump pressurization to induce bulk flow. This system takes advantage of the increased amount of antibody immobilized on the 3D microfiber and the accelerated propagation of antigens through the microfiber induced by the vacuum pump to enable an efficient and rapid immunoassay.Highly sensitive and rapid biosensing on a three-dimensional polymer platform. Increasing sensitivity and decreasing assay time are two of the most crucial goals in the development of biosensing devices. We have developed three-dimensional (3D) nanospherical and microfiber structure for biosensing devices aimed at highly sensitive and rapid immunoassays. The system having polystyrene microfiber of 3D structure paired with vacuum pump pressurization to induce bulk flow enables an efficient and rapid immunoassay. Because it takes advantage of the increased amount of immobilized antibody on 3D structure and of the accelerated propagation of antigens by flowing through the microfiber.

中文翻译：

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三维聚合物平台上的高灵敏度和快速生物传感

摘要 提高灵敏度和缩短检测时间是生物传感设备（如免疫检测、蛋白质电泳和生物成像纳米探针）开发中最重要的两个目标。我们已经为生物传感设备开发了三维 (3D) 纳米/微结构平台，用于高度灵敏和快速的免疫测定。一个这样的平台是具有聚[2-甲基丙烯酰氧基乙基磷酰胆碱-共-甲基丙烯酸正丁酯-共-对硝基苯氧羰基聚（乙二醇）甲基丙烯酸酯]（PMBN）的纳米球结构，该结构通过电喷雾沉积（ESD）方法制备。与平面 PMBN 相比，3D 纳米球表面能够固定大量抗体，从而增强免疫测定的特异性信号。此外，PMBN 抗非特异性蛋白质吸附。因此，这种 3D 纳米球结构可应用于微芯片环境，从而实现高度灵敏和快速的免疫测定。为了获得最多需要 5 分钟的更快速检测，我们使用了另一个平台：由 ESD 制备的 3D 聚苯乙烯 (PS) 微纤维结构与真空泵加压相结合以诱导大量流动。该系统利用固定在 3D 超细纤维上的抗体数量增加以及真空泵诱导抗原通过超细纤维的加速传播来实现高效快速的免疫测定。在三维聚合物平台上实现高灵敏度和快速生物传感。提高灵敏度和缩短检测时间是生物传感设备开发中最重要的两个目标。我们开发了三维 (3D) 纳米球和微纤维结构，用于生物传感设备，旨在高度灵敏和快速的免疫测定。该系统具有 3D 结构的聚苯乙烯微纤维，搭配真空泵加压以诱导大量流动，可实现高效快速的免疫测定。因为它利用了 3D 结构上固定化抗体数量的增加和抗原通过微纤维流动的加速传播。