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Analysis of binding properties of pathogens and toxins using multivalent glycan microarrays†
RSC Advances ( IF 3.9 ) Pub Date : 2018-04-19 00:00:00 , DOI: 10.1039/c8ra01285g Hyoung Sub Kim 1 , Ji Young Hyun 1 , Seong-Hyun Park 1 , Injae Shin 1
RSC Advances ( IF 3.9 ) Pub Date : 2018-04-19 00:00:00 , DOI: 10.1039/c8ra01285g Hyoung Sub Kim 1 , Ji Young Hyun 1 , Seong-Hyun Park 1 , Injae Shin 1
Affiliation
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Pathogens infect hosts often through initial binding of their cell surface lectins to glycans expressed on the exterior of host cells. Thus, methods to evaluate the glycan-binding properties of pathogens are of great importance. Because of the multivalent nature of interactions of pathogens with glycans, the ability to assess the glycan density-dependent binding of pathogens is particularly important. In this study, we developed a facile technique to construct multivalent carbohydrate microarrays through immobilization of unmodified glycans on multivalent hydrazide-derivatized glass surfaces. This immobilization strategy does not require the use of multivalent glycoconjugates, which are typically prepared by using multistep sequences. The results of analysis of microarray images, obtained after incubation of multivalent glycan microarrays with cholera toxin B and pathogens such as uropathogenic E. coli and H. pylori, show that the binding affinities of toxins and pathogens for glycans are highly glycan density-dependent. Specifically, toxins and pathogens bind to glycans more strongly as the valency of the glycans on the microarrays is increased from 1 to 4. It is anticipated that the newly developed immobilization method will be applicable to the preparation of multivalent carbohydrate microarrays that are employed to evaluate multivalent glycan binding properties of a variety of pathogens and toxins.
中文翻译：
使用多价聚糖微阵列分析病原体和毒素的结合特性†
病原体通常通过其细胞表面凝集素与宿主细胞外部表达的聚糖的初始结合来感染宿主。因此，评估病原体聚糖结合特性的方法非常重要。由于病原体与聚糖相互作用的多价性质，评估病原体与聚糖密度依赖性结合的能力尤为重要。在这项研究中，我们开发了一种简便的技术，通过在多价酰肼衍生玻璃表面上固定未修饰的聚糖来构建多价碳水化合物微阵列。这种固定策略不需要使用多价糖缀合物，它们通常是通过使用多步序列制备的。微阵列图像的分析结果，E. coli和H. pylori表明，毒素和病原体对聚糖的结合亲和力高度依赖于聚糖密度。具体而言，随着微阵列上聚糖的化合价从 1 增加到 4，毒素和病原体与聚糖的结合更强烈。预计新开发的固定化方法将适用于制备用于评估的多价碳水化合物微阵列多种病原体和毒素的多价聚糖结合特性。
更新日期：2018-04-19
中文翻译：
使用多价聚糖微阵列分析病原体和毒素的结合特性†
病原体通常通过其细胞表面凝集素与宿主细胞外部表达的聚糖的初始结合来感染宿主。因此，评估病原体聚糖结合特性的方法非常重要。由于病原体与聚糖相互作用的多价性质，评估病原体与聚糖密度依赖性结合的能力尤为重要。在这项研究中，我们开发了一种简便的技术，通过在多价酰肼衍生玻璃表面上固定未修饰的聚糖来构建多价碳水化合物微阵列。这种固定策略不需要使用多价糖缀合物，它们通常是通过使用多步序列制备的。微阵列图像的分析结果，E. coli和H. pylori表明，毒素和病原体对聚糖的结合亲和力高度依赖于聚糖密度。具体而言，随着微阵列上聚糖的化合价从 1 增加到 4，毒素和病原体与聚糖的结合更强烈。预计新开发的固定化方法将适用于制备用于评估的多价碳水化合物微阵列多种病原体和毒素的多价聚糖结合特性。
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