Magnetic interactions and in vitro study of biocompatible hydrocaffeic acid-stabilized Fe–Pt clusters as MRI contrast agents†,RSC Advances

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Magnetic interactions and in vitro study of biocompatible hydrocaffeic acid-stabilized Fe–Pt clusters as MRI contrast agents†
RSC Advances ( IF 3.9 ) Pub Date : 2018-04-19 00:00:00 , DOI: 10.1039/c8ra00047f
N Kostevšek ₁ , S Hudoklin ₂ , M E Kreft ₂ , I Serša ₃ , A Sepe ₃ , Z Jagličić ₄ , J Vidmar ₅ , J Ščančar ₅ , S Šturm _{1,

6} , S Kobe _{1,

6} , K Žužek Rožman _{1,

6}

Affiliation

A detailed magnetic study of separated Fe–Pt NPs and Fe–Pt clusters was performed to predict their optimal size and morphology for the maximum saturation magnetization, a factor that is known to influence the performance of a magnetic-resonance-imaging (MRI) contrast agent. Excellent stability and biocompatibility of the nanoparticle suspension was achieved using a novel coating based on hydrocaffeic acid (HCA), which was confirmed with a detailed Fourier-transform infrared spectroscopy (FTIR) study. An in vitro study on a human-bladder papillary urothelial neoplasm RT4 cell line confirmed that HCA-Fe–Pt nanoparticles showed no cytotoxicity, even at a very high concentration (550 μg Fe–Pt per mL), with no delayed cytotoxic effect being detected. This indicates that the HCA coating provides excellent biocompatibility of the nanoparticles, which is a prerequisite for the material to be used as a safe contrast agent for MRI. The cellular uptake and internalization mechanism were studied using ICP-MS and TEM analyses. Furthermore, it was shown that even a very low concentration of Fe–Pt nanoparticles (<10 μg mL⁻¹) in the cells is enough to decrease the T₂ relaxation times by 70%. In terms of the MRI imaging, this means a large improvement in the contrast, even at a low nanoparticle concentration and an easier visualization of the tissues containing nanoparticles, proving that HCA-coated Fe–Pt nanoparticles have the potential to be used as an efficient and safe MRI contrast agent.

中文翻译：

生物相容性氢咖啡酸稳定的 Fe-Pt 簇作为 MRI 造影剂的磁相互作用和体外研究†

对分离的 Fe-Pt NPs 和 Fe-Pt 簇进行了详细的磁性研究，以预测其最大饱和磁化强度的最佳尺寸和形态，这是已知会影响磁共振成像 (MRI) 对比度性能的因素代理人。使用基于氢咖啡酸 (HCA) 的新型涂层实现了纳米颗粒悬浮液的出色稳定性和生物相容性，详细的傅里叶变换红外光谱 (FTIR) 研究证实了这一点。体外_对人膀胱乳头状尿路上皮肿瘤 RT4 细胞系的研究证实，HCA-Fe-Pt 纳米粒子即使在非常高的浓度（550 μg Fe-Pt/mL）下也没有显示出细胞毒性，也没有检测到延迟的细胞毒性作用。这表明 HCA 涂层为纳米粒子提供了优异的生物相容性，这是该材料用作 MRI 安全造影剂的先决条件。使用 ICP-MS 和 TEM 分析研究了细胞摄取和内化机制。此外，结果表明，即使细胞中浓度非常低的 Fe-Pt 纳米颗粒 (<10 μg mL ^-1 ) 也足以降低T ₂松弛时间减少 70%。就 MRI 成像而言，这意味着即使在低纳米颗粒浓度下对比度也有很大提高，并且含有纳米颗粒的组织更容易可视化，这证明了 HCA 包覆的 Fe-Pt 纳米颗粒有可能用作有效的和安全的MRI造影剂。

更新日期：2018-04-19

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