Identification of a novel aminopolycarboxylic acid siderophore gene cluster encoding the biosynthesis of ethylenediaminesuccinic acid hydroxyarginine (EDHA)†,Metallomics

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Identification of a novel aminopolycarboxylic acid siderophore gene cluster encoding the biosynthesis of ethylenediaminesuccinic acid hydroxyarginine (EDHA)†
Metallomics ( IF 2.9 ) Pub Date : 2018-04-18 00:00:00 , DOI: 10.1039/c8mt00009c
Marius Spohn _{1,

2,

3,

4,

5} , Simone Edenhart _{1,

2,

3,

4,

5} , Mohammad Alanjary _{1,

2,

3,

4,

5} , Nadine Ziemert _{1,

2,

3,

4,

5} , Daniel Wibberg _{5,

6,

7,

8,

9} , Jörn Kalinowski _{5,

6,

7,

8,

9} , Timo H. J. Niedermeyer _{1,

2,

3,

4,

5} , Evi Stegmann _{1,

2,

3,

4,

5} , Wolfgang Wohlleben _{1,

2,

3,

4,

5}

Affiliation

The mechanism of siderophore-mediated iron supply enhances fitness and survivability of microorganisms under iron limited growth conditions. One class of naturally occurring ionophores is the small aminopolycarboxylic acids (APCAs). Although they are structurally related to the most famous anthropogenic chelating agent, ethylenediaminetetraacetate (EDTA), they have been largely neglected by the scientific community. Here, we demonstrate the detection of APCA gene clusters by a computational screening of a nucleotide database. This genome mining approach enabled the discovery of a yet unknown APCA gene cluster in well-described actinobacterial strains, either known for their potential to produce valuable secondary metabolites (Streptomyces avermitilis) or for their pathogenic lifestyle (Streptomyces scabies, Corynebacterium pseudotuberculosis, Corynebacterium ulcerans and Nocardia brasiliensis). The herein identified gene cluster was shown to encode the biosynthesis of APCA, ethylenediaminesuccinic acid hydroxyarginine (EDHA). Detailed and comparatively performed production and transcriptional profiling of EDHA and its biosynthesis genes showed strict iron-responsive biosynthesis.

中文翻译：

鉴定编码乙二胺琥珀酸羟基精氨酸（EDHA）生物合成的新型氨基聚羧酸铁载体基因簇†

在铁受限的生长条件下，铁载体介导的铁供应机制增强了微生物的适应性和生存能力。一类天然存在的离子载体是小的氨基聚羧酸（APCA）。尽管它们在结构上与最著名的人为螯合剂乙二胺四乙酸酯（EDTA）有关，但它们在很大程度上已被科学界所忽略。在这里，我们通过核苷酸数据库的计算筛选证明了APCA基因簇的检测。这种基因组挖掘方法使人们能够在描述良好的放线菌菌株中发现一个未知的APCA基因簇，该菌株以其产生有价值的次级代谢产物（阿维链霉菌）的潜力或其致病性生活方式而闻名（链霉菌sc疮，假结核棒杆菌，溃疡棒杆菌和巴西诺卡氏菌）。显示本文中鉴定的基因簇编码APCA，乙二胺琥珀酸羟基精氨酸（EDHA）的生物合成。EDHA及其生物合成基因的详细和比较执行的生产和转录谱显示严格的铁反应性生物合成。

更新日期：2018-04-18

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