The phytochemical study of Euphorbia resinifera afforded 18 structurally diverse diterpenoids, including 14 new ingol-type diterpenoids, euphorblins A–N (1–14), a new rhamnofolane diterpenoid, euphorblin O (15), and three known analogues (16–18). The structures of these compounds were deduced using 2D NMR spectroscopy and NOE experiments. The structure of compound 1 was confirmed by single-crystal X-ray crystallography. The abilities of the compounds to enhance lysosomal biosynthesis were evaluated through LysoTracker Red staining. Among the 10 active compounds, compounds 2, 4, and 18 showed remarkable immunofluorescence strength, and their LysoTracker staining intensities were 155.9%, 143.5%, and 140.7%, respectively, greater than that of the control. A series of lysosomal genes were also found to be upregulated by these compounds, which further confirms their ability to induce lysosome biosynthesis and suggests that these diterpenoids have potential as lead compounds for the development of drugs for the treatment of lysosome-related diseases.

中文翻译：

---

大戟树脂中英戈尔和鼠李糖醇双萜的鉴定及其诱导溶酶体生物合成的能力

的植物化学研究大戟resinifera得到18个结构不同的二萜类化合物，其中包括14个新的ingol型二萜，euphorblins A-N（1 - 14），一个新的rhamnofolane二萜，euphorblin O（15），和三种已知的类似物（16 - 18） 。使用2D NMR光谱和NOE实验推导了这些化合物的结构。化合物1的结构通过单晶X射线晶体学确认。通过LysoTracker Red染色评估化合物增强溶酶体生物合成的能力。间10的活性化合物，化合物2，4，和18显示出显着的免疫荧光强度，其LysoTracker染色强度分别比对照组高155.9％，143.5％和140.7％。还发现一系列溶酶体基因被这些化合物上调，这进一步证实了它们诱导溶酶体生物合成的能力，并表明这些二萜具有作为开发治疗溶酶体相关疾病的药物的先导化合物的潜力。