Evaluation of Natural and Synthetic Phosphate Donors for the Improved Enzymatic Synthesis of Phosphate Monoesters,Advanced Synthesis & Catalysis

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Evaluation of Natural and Synthetic Phosphate Donors for the Improved Enzymatic Synthesis of Phosphate Monoesters
Advanced Synthesis & Catalysis ( IF 4.4 ) Pub Date : 2018-05-02 , DOI: 10.1002/adsc.201800306
Gábor Tasnádi _{1,

2} , Wolfgang Jud ₂ , Mélanie Hall ₂ , Kai Baldenius ₃ , Klaus Ditrich ₃ , Kurt Faber ₂

Affiliation

Undesired product hydrolysis along with large amounts of waste in form of inorganic monophosphate by‐product are the main obstacles associated with the use of pyrophosphate in the phosphatase‐catalyzed synthesis of phosphate monoesters on large scale. In order to overcome both limitations, we screened a broad range of natural and synthetic organic phosphate donors with several enzymes on a broad variety of hydroxyl‐compounds. Among them, acetyl phosphate delivered stable product levels and high phospho‐transfer efficiency at the lower functional pH‐limit, which translated into excellent productivity. The protocol is generally applicable to acid phosphatases and compatible with a range of diverse substrates. Preparative‐scale transformations using acetyl phosphate synthesized from cheap starting materials yielded multiple grams of various sugar phosphates with up to 433 g L⁻¹ h⁻¹ space‐time yield and 75% reduction of barium phosphate waste.

中文翻译：

天然和合成磷酸盐供体对改进磷酸单酯酶法合成的评价

不期望的产物水解以及大量无机单磷酸酯副产物形式的废物是在磷酸酶催化的磷酸单酯大规模合成中使用焦磷酸盐的主要障碍。为了克服这两个限制，我们用多种酶对多种羟基化合物筛选了多种天然和合成的有机磷酸盐供体。其中，乙酰磷酸在较低的功能 pH 限制下提供稳定的产品水平和高磷酸转移效率，这转化为出色的生产率。该方案通常适用于酸性磷酸酶，并与一系列不同的底物兼容。使用廉价起始材料合成的乙酰磷酸盐进行制备规模转化，产生了多克各种糖磷酸盐，时空产率高达 433 g L ^-1 h ^-1 ，并且磷酸钡废物减少了 75%。

更新日期：2018-05-02

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