Recombinant spider silk has arisen as one of the most promising nature-derived building blocks owing to its extraordinary mechanical properties, while its instability against thermo-oxidative degradation becomes a major drawback toward industrial applications. Here, we have firstly implemented a high-throughput screening of antioxidants on the stabilization of recombinant spider silk at an elevated temperature. The usage of high-throughput chemiluminescence imaging has allowed us to screen antioxidants with a wide spectrum of molecular structures and quickly provided two good candidates to stabilize recombinant spider silk powder through an impregnation process: E310 and BHT. An accelerated aging has further proven that these antioxidants suppressed the thermo-oxidation of recombinant spider silk through scavenging the formed radical species and slowed down the formation of carbonyl groups as oxidation products. In addition, we have employed a solution mixing process to further improve the stabilization efficacy of the antioxidants and this method also extended our selection on effective antioxidants, including vitamin E, AO-30, AO-40, HP-10, and especially Irganox 1098. These screening results provide guidelines of selecting or even developing potential antioxidants for stabilizing protein materials.

重组蜘蛛丝由于其非凡的机械性能而已成为最有前途的自然衍生构件之一，而其抗热氧化降解的不稳定性成为工业应用的主要缺点。在这里，我们首先在高温下对重组蜘蛛丝的稳定化进行了抗氧化剂的高通量筛选。高通量化学发光成像的使用使我们能够筛选具有广泛分子结构的抗氧化剂，并迅速提供了两个很好的候选物来通过浸渍过程稳定重组蜘蛛丝粉末：E310和BHT。加速老化进一步证明，这些抗氧化剂通过清除形成的自由基种类并抑制作为氧化产物的羰基的形成，抑制了重组蜘蛛丝的热氧化。此外，我们采用溶液混合工艺进一步提高了抗氧化剂的稳定效果，该方法还扩展了我们对有效抗氧化剂的选择，包括维生素E，AO-30，AO-40，HP-10，尤其是Irganox 1098这些筛选结果为选择或开发潜在的抗氧化剂以稳定蛋白质材料提供了指导。