Abstract Disposable screen printed carbon electrodes have been developed for the quantitative determination of free bilirubin. The electrodes were fabricated using graphite carbon ink and characterized with microscopy, spectroscopy and diffraction studies. The carbon ink for printing is made of graphite nanoparticles of size around 50 nm. Electrochemical oxidation of bilirubin was carried out voltammetrically in Trizma buffer of pH 8.5. The sensor showed a dynamic detection range of 5–600 μM and a sensitivity of 95 μAμM−1 cm−2. The SPCE showed superior performance than many of the reported sensors in terms of dynamic range, sensitivity and oxidation potential. Molecules such as ascorbic acid, uric acid, dopamine, glucose, creatinine and ethanol were tested using SPCE and found to be non interfering with the detection of creatinine in the physiological conditions. The sensor was tested using bilirubin spiked serum samples and found that it is giving response to free bilirubin. The sensor was also used to study the affinity of free bilirubin to albumin and displacement of bilirubin from albumin by ibuprofen.

中文翻译：

---

一次性丝网印刷碳电极伏安法测定胆红素

摘要 一次性丝网印刷碳电极已被开发用于游离胆红素的定量测定。电极是使用石墨碳墨水制造的，并通过显微镜、光谱和衍射研究进行表征。用于印刷的碳墨由尺寸约为 50 nm 的石墨纳米颗粒制成。胆红素的电化学氧化在 pH 8.5 的 Trizma 缓冲液中以伏安法进行。该传感器的动态检测范围为 5–600 μM，灵敏度为 95 μAμM-1 cm-2。SPCE 在动态范围、灵敏度和氧化电位方面表现出优于许多报道的传感器的性能。分子如抗坏血酸、尿酸、多巴胺、葡萄糖、肌酐和乙醇使用 SPCE 进行测试，发现在生理条件下不干扰肌酐的检测。该传感器使用加标胆红素的血清样品进行测试，发现它对游离胆红素有反应。该传感器还用于研究游离胆红素与白蛋白的亲和力以及布洛芬从白蛋白中置换胆红素。