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Ginnalin A from Kujin tea (Acer tataricum subsp. ginnala) exhibits a colorectal cancer chemoprevention effect via activation of the Nrf2/HO-1 signaling pathway†
Food & Function ( IF 5.1 ) Pub Date : 2018-04-17 00:00:00 , DOI: 10.1039/c8fo00054a Wu Bi 1, 2, 3, 4, 5 , Chun-nian He 6, 7, 8, 9, 10 , Xiao-xiao Li 1, 2, 3, 4, 5 , Liu-ying Zhou 1, 2, 3, 4, 5 , Rui-jie Liu 1, 2, 3, 4, 5 , Sai Zhang 1, 2, 3, 4, 5 , Guo-qing Li 11, 12, 13, 14, 15 , Zhu-chu Chen 1, 2, 3, 4, 5 , Peng-fei Zhang 1, 2, 3, 4, 5
Food & Function ( IF 5.1 ) Pub Date : 2018-04-17 00:00:00 , DOI: 10.1039/c8fo00054a Wu Bi 1, 2, 3, 4, 5 , Chun-nian He 6, 7, 8, 9, 10 , Xiao-xiao Li 1, 2, 3, 4, 5 , Liu-ying Zhou 1, 2, 3, 4, 5 , Rui-jie Liu 1, 2, 3, 4, 5 , Sai Zhang 1, 2, 3, 4, 5 , Guo-qing Li 11, 12, 13, 14, 15 , Zhu-chu Chen 1, 2, 3, 4, 5 , Peng-fei Zhang 1, 2, 3, 4, 5
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Ginnalin A (also known as acertannin) is one of the most important phenolic compounds of several beverage Acer plants. In this study, it is reported for the first time that ginnalin A is an activator of the Nrf2 signaling pathway in human colon cancer cells. Ginnalin A, isolated from the leaves of Acer tataricum subsp. ginnala, exhibited promising preventive activity against colon cancer cells (HCT116, SW480 and SW620) with IC50 values of 24.8 μM, 22.0 μM and 39.7 μM, respectively. In addition, it significantly reduced the colony formation of these cells. Flow cytometry analysis indicated that ginnalin A suppressed cancer proliferation via the induction of cell cycle arrest at the S-phase. Real time PCR analysis demonstrated that ginnalin A can upregulate the mRNA expression levels of Nrf2-related antioxidant genes Nrf2, HO-1 and NQO1. Western blotting analysis revealed that ginnalin A promoted the Nrf2 nuclear translocation and upregulated the proteins Nrf2, HO-1 and NQO1. Moreover, the upregulation of p62 and the inhibition of Keap1 were also found by Western blotting analysis. Therefore, the activation of the Nrf2 signaling pathway was probably induced through the upregulation of p62 and the inhibition of Keap1.
中文翻译：
从酷劲茶Ginnalin A（宏基苦荞麦亚种茶条）表现出结直肠癌化学预防效果经由所述Nrf2 / HO-1信号传导途径的活化†
Ginnalin A（又称天青素）是几种Acer饮料植物中最重要的酚类化合物之一。在这项研究中，首次报道了人参醛A是人结肠癌细胞中Nrf2信号通路的激活剂。Ginnalin A，从槭树亚种的叶子中分离。银杏（Ginnala）对结肠癌细胞（HCT116，SW480和SW620）表现出有希望的预防活性，其IC 50值分别为24.8μM，22.0μM和39.7μM。另外，它显着减少了这些细胞的集落形成。流式细胞仪分析表明，人参皂苷A通过以下方式抑制了癌症的扩散诱导细胞周期停滞在S期。实时PCR分析表明，人参醛A可以上调与Nrf2相关的抗氧化基因Nrf2，HO-1和NQO1的mRNA表达水平。蛋白质印迹分析表明，人参醛A促进Nrf2核易位并上调蛋白Nrf2，HO-1和NQO1。此外，还通过蛋白质印迹分析发现了p62的上调和对Keap1的抑制。因此，可能通过上调p62和抑制Keap1来诱导Nrf2信号通路的激活。
更新日期：2018-04-17
中文翻译：
从酷劲茶Ginnalin A（宏基苦荞麦亚种茶条）表现出结直肠癌化学预防效果经由所述Nrf2 / HO-1信号传导途径的活化†
Ginnalin A（又称天青素）是几种Acer饮料植物中最重要的酚类化合物之一。在这项研究中，首次报道了人参醛A是人结肠癌细胞中Nrf2信号通路的激活剂。Ginnalin A，从槭树亚种的叶子中分离。银杏（Ginnala）对结肠癌细胞（HCT116，SW480和SW620）表现出有希望的预防活性，其IC 50值分别为24.8μM，22.0μM和39.7μM。另外，它显着减少了这些细胞的集落形成。流式细胞仪分析表明，人参皂苷A通过以下方式抑制了癌症的扩散诱导细胞周期停滞在S期。实时PCR分析表明，人参醛A可以上调与Nrf2相关的抗氧化基因Nrf2，HO-1和NQO1的mRNA表达水平。蛋白质印迹分析表明，人参醛A促进Nrf2核易位并上调蛋白Nrf2，HO-1和NQO1。此外，还通过蛋白质印迹分析发现了p62的上调和对Keap1的抑制。因此，可能通过上调p62和抑制Keap1来诱导Nrf2信号通路的激活。
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