Voltage‐gated Ca²⁺ (Ca_V) channels mediate Ca²⁺ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca_V channel modulators, to enable photo‐tunable modulation of Ca_V channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca_V channels could find broad applications in interrogating a wide range of Ca_V‐mediated physiological processes.

中文翻译：

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电压门控钙通道的光遗传控制

电压门控的Ca ²⁺（Ca _V）通道介导Ca ²⁺进入兴奋性细胞，从而调节膜去极化后的大量细胞事件。我们报告了RGK GTPases的工程设计，这是一类遗传编码的Ca _V通道调节剂，能够在可激发的哺乳动物细胞中对Ca _V通道活性进行光可调的调节。为Ca _V通道量身定制的这种光遗传学工具（指定为optoRGK）可以在询问广泛的Ca _V介导的生理过程中找到广泛的应用。