Synthesis and effect of a new Terbium gibberellic complex on the histopathological alteration induced by Gibberellic acid on liver and kidney of mice Mus musculus,Chemical Biology & Drug Design

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Synthesis and effect of a new Terbium gibberellic complex on the histopathological alteration induced by Gibberellic acid on liver and kidney of mice Mus musculus
Chemical Biology & Drug Design ( IF 3.2 ) Pub Date : 2018-04-16 , DOI: 10.1111/cbdd.13191
Amin A. Seleem _{1,

2} , Belal H. M. Hussein _{3,

4}

Affiliation

The objective of this study was to synthesize Gibberellic lanthanide complex and evaluate its biological activity to reduce the Gibberellic acid toxicity on liver and kidney. The new bis(Gibberellic)‐nitro‐terbium(III) complex was characterized by different analytical methods: elemental analyses, UV–Vis, molar ratio, fluorescence, FT‐IR, and TGA‐DTA measurements. Thirty newborns were classified into three groups control, Gibberellic acid, and Terbium gibberellic acid complex. Livers and kidneys of studied groups proceed for general histology and immunohistochemical staining of Cyr61, cytochrome C, and TNFR2. From the absorption titration measurements, the binding constants of DNA with Tb(III)‐(GA)₂ complex and free ligand were found to be 3.9 × 10⁴ and 2.1 × 10³ m⁻¹, respectively, with the stoichiometry of 1:1. Hypochromism was observed from the absorption titration experiment which indicates the intercalation of Tb(III)‐(GA)₂ complex between the base pairs of DNA. Gibberellic acid‐treated group showed alteration in the histological picture of livers and kidneys that accompanied with the reduction in the expression of Cyr61, cyt C, and TNFR2. The amelioration was observed in Gibberellic acid complex with Terbium group. The study concluded that Terbium gibberellic complex is less dangerous effects than Gibberellic acid alone.

中文翻译：

新型赤霉素复合物的合成及其对赤霉素对小鼠小家鼠肝肾的组织病理学改变的影响

这项研究的目的是合成赤霉素镧系元素复合物，并评估其生物活性，以减少赤霉素对肝脏和肾脏的毒性。新的双（赤霉素）-硝基‐（III）络合物的特征在于采用了不同的分析方法：元素分析，UV-Vis，摩尔比，荧光，FT-IR和TGA-DTA测量。30例新生儿分为三组：赤霉素和Ter赤霉酸复合物。研究组的肝脏和肾脏进行Cyr61，细胞色素C和TNFR2的一般组织学和免疫组织化学染色。通过吸收滴定测量，发现DNA与Tb（III）-（GA）₂复合物和游离配体的结合常数为3.9×10 ^4。和2.1×10 ³ m ^-1，化学计量比为1：1。从吸收滴定实验中观察到了变色现象，这表明Tb（III）-（GA）_2配合物在DNA碱基对之间的插入。赤霉素治疗组肝脏和肾脏的组织学改变，并伴有Cyr61，cyt C和TNFR2表达的降低。赤霉酸与Ter基配合物观察到了改善。研究得出的结论是，Ter赤霉素复合物的危险性比单独使用赤霉酸的危险性小。

更新日期：2018-04-16

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