Sticky-flares for in situ monitoring of human telomerase RNA in living cells†,Nanoscale

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Sticky-flares for in situ monitoring of human telomerase RNA in living cells†
Nanoscale ( IF 5.8 ) Pub Date : 2018-04-17 00:00:00 , DOI: 10.1039/c8nr01260a
Qilong Wu _{1,

2,

3,

4,

5} , Zhengjie Liu _{1,

2,

3,

4,

5} , Lei Su _{1,

2,

3,

4,

5} , Guangmei Han _{1,

2,

3,

4,

5} , Renyong Liu _{1,

2,

3,

4,

5} , Jun Zhao _{1,

2,

3,

4} , Tingting Zhao _{1,

2,

3,

4} , Changlong Jiang _{1,

2,

3,

4} , Zhongping Zhang _{1,

2,

3,

4,

6}

Affiliation

Human telomerase RNA (hTR), a template of telomerase for telomeric repeat synthesis, was used to reflect the telomerase activity and act as a potential target of antitumor therapy. Here, we report a novel DNA-conjugated AuNP probe termed sticky-flares for the in situ detection of intracellular human telomerase RNA. The sticky-flares probe is capable of entering living cells directly without any auxiliary and recognizing the binding domain of human telomerase RNA. On recognition, the fluorophore-modified recognition flares can specifically bind to the target, separate from the sticky-flares and act as a fluorescent reporter to quantify and dynamically profile human telomerase RNA in living cells. We envision that the sticky-flares probe would be a valuable platform to investigate the function and regulation of hTR in antitumor therapy and hTR-related drug invention.

中文翻译：

用于在活细胞中原位监测人端粒酶RNA的粘滞弹头†

人端粒酶RNA（hTR）是端粒重复合成的端粒酶模板，用于反映端粒酶活性并充当抗肿瘤治疗的潜在靶标。这里，我们报告一个新的DNA-缀合的AuNP探针称为粘性耀斑的原位细胞内人端粒酶RNA的检测。粘滞耀斑探针能够在没有任何辅助的情况下直接进入活细胞并识别人端粒酶RNA的结合域。识别后，荧光团修饰的识别光斑可以与靶标特异性结合，与粘性光斑分开，并充当荧光报告基因，以定量和动态分析活细胞中的人类端粒酶RNA。我们设想，粘滞光斑探针将是研究hTR在抗肿瘤治疗和hTR相关药物发明中的功能和调控的有价值的平台。

更新日期：2018-04-17

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