A split aptamer-based imaging solution for the visualization of latent fingerprints†,Analytical Methods

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A split aptamer-based imaging solution for the visualization of latent fingerprints†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-04-16 00:00:00 , DOI: 10.1039/c8ay00538a
Xiaojuan Liu _{1,

2,

3,

4} , Xiuyuan Li _{1,

2,

3,

4} , Yingying Lu _{1,

2,

3,

4} , Jiabing Cao _{1,

2,

3,

4} , Feng Li _{1,

2,

3,

4}

Affiliation

An efficient method for detecting latent fingerprints (LFPs) is very important for personal identification in forensic science. Herein, a simple label-free strategy was designed for the visualization of LFPs by using a split aptamer-based imaging solution. The imaging solution consists of N-methyl mesoporphyrin IX (NMM) molecules and two DNA strands both embedded with split aptamers and split G-quadruplex sequences, respectively. The split aptamers were used to specifically bind with the lysozyme in the residues of LFPs, which brought the two DNA strands into proximity and resulted in the formation of a G-quadruplex/NMM complex. Taking advantage of the high recognition power of the aptamers and the unique fluorescence feature of the G-quadruplex/NMM complex, this split aptamer-based imaging solution was successfully applied for the visualization of LFPs under the irradiation of a handheld UV lamp. Furthermore, this approach was robust for visualizing LFPs on different substrates and from different volunteers. Therefore, this LFP imaging strategy might find wide application in forensic investigations.

中文翻译：

基于拆分的适体成像解决方案，可用于潜在指纹的可视化†

检测潜在指纹（LFP）的有效方法对于法医学中的个人识别非常重要。本文中，通过使用基于适体的拆分成像解决方案，设计了一种简单的无标签策略来可视化LFP。成像解决方案包含N-甲基中卟啉IX（NMM）分子和两条DNA链均分别嵌入了分裂的适体和分裂的G-四链体序列。分裂的适体用于与LFP残基中的溶菌酶特异性结合，使两条DNA链接近并导致G-四链体/ NMM复合体的形成。利用适体的高识别能力和G-四链体/ NMM复合体的独特荧光特征，这种基于分离的适体的成像解决方案已成功应用于手持式紫外灯照射下LFP的可视化。此外，这种方法对于在不同基材上以及来自不同志愿者的LFP可视化方面非常可靠。因此，这种LFP成像策略可能在法医调查中得到广泛应用。

更新日期：2018-04-16

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