Fluorogenic Ag+–Tetrazolate Aggregation Enables Efficient Fluorescent Biological Silver Staining,Angewandte Chemie International Edition

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Fluorogenic Ag+–Tetrazolate Aggregation Enables Efficient Fluorescent Biological Silver Staining
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-04-14 , DOI: 10.1002/anie.201801653
Sheng Xie _{1,

2} , Alex Y H Wong ₂ , Ryan T K Kwok ₁ , Ying Li ₁ , Huifang Su ₁ , Jacky W Y Lam ₁ , Sijie Chen ₂ , Ben Zhong Tang _{1,

3,

4}

Affiliation

Silver staining, which exploits the special bioaffinity and the chromogenic reduction of silver ions, is an indispensable visualization method in biology. It is a most popular method for in‐gel protein detection. However, it is limited by run‐to‐run variability, background staining, inability for protein quantification, and limited compatibility with mass spectroscopic (MS) analysis; limitations that are largely attributed to the tricky chromogenic visualization. Herein, we reported a novel water‐soluble fluorogenic Ag⁺ probe, the sensing mechanism of which is based on an aggregation‐induced emission (AIE) process driven by tetrazolate‐Ag⁺ interactions. The fluorogenic sensing can substitute the chromogenic reaction, leading to a new fluorescence silver staining method. This new staining method offers sensitive detection of total proteins in polyacrylamide gels with a broad linear dynamic range and robust operations that rival the silver nitrate stain and the best fluorescent stains.

中文翻译：

荧光 Ag+-四唑盐聚集可实现高效的荧光生物银染色

银染色利用银离子特殊的生物亲和力和显色还原作用，是生物学中不可或缺的可视化方法。它是凝胶内蛋白质检测最流行的方法。然而，它受到运行间变异性、背景染色、无法定量蛋白质以及与质谱 (MS) 分析的有限兼容性的限制；其局限性很大程度上归因于棘手的显色可视化。在此，我们报道了一种新型水溶性荧光Ag ⁺探针，其传感机制基于四唑盐-Ag ⁺相互作用驱动的聚集诱导发射（AIE）过程。荧光传感可以替代显色反应，产生一种新的荧光银染色方法。这种新的染色方法可以灵敏地检测聚丙烯酰胺凝胶中的总蛋白，具有宽广的线性动态范围和稳健的操作，可与硝酸银染色剂和最佳荧光染色剂相媲美。

更新日期：2018-04-14

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