Ionic liquid dissolution/precipitation pretreatment of barley straw was used to improve the yield to produce ethanol. The pretreatment with ionic liquid greatly improves the enzymatic hydrolysis compared to untreated samples, reducing the hydrolysis time to only 24 h, which is lower than untreated samples and samples treated with other methods. The treatment with 1-ethyl-3-methylimidazolium acetate produced a better hydrolysis yield with a glucose yield close to 100%. We produced ethanol with pretreated samples using simultaneous saccharification and fermentation (SSF) and presaccharification, saccharification, and fermentation (PSSF). In both procedures we obtained very high and similar yields because the pretreatment enhances the enzymatic hydrolysis rate, and no presaccharification is needed. We obtained 22.9 g ethanol/100 g pretreated barley straw, representing 97% of the potential ethanol from the cellulose, one of the highest yields reported in the literature.

中文翻译：

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IL预处理的大麦秸秆同时发酵与糖化相结合的第二代生物乙醇生产

大麦秸秆的离子液体溶解/沉淀预处理可提高产率，以生产乙醇。与未处理的样品相比，用离子液体进行的预处理大大改善了酶促水解，将水解时间缩短至仅24小时，这比未处理的样品和其他方法处理的样品要短。用乙酸1-乙基-3-甲基咪唑鎓的处理产生更好的水解产率，葡萄糖产率接近100％。我们使用同时​​进行的糖化和发酵（SSF）和预糖化，糖化和发酵（PSSF）的预处理样品生产了乙醇。在这两种方法中，由于预处理提高了酶水解速率，并且不需要进行预糖化，因此我们获得了非常高且相似的产率。我们获得了22。