Reprogramming Probiotic Lactobacillus reuteri as a Biosensor for Staphylococcus aureus Derived AIP-I Detection,ACS Synthetic Biology

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Reprogramming Probiotic Lactobacillus reuteri as a Biosensor for Staphylococcus aureus Derived AIP-I Detection
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-04-13 00:00:00 , DOI: 10.1021/acssynbio.8b00063
David Lubkowicz _{1,

2} , Chun Loong Ho _{1,

2} , In Young Hwang _{1,

2} , Wen Shan Yew _{1,

2} , Yung Seng Lee _{2,

3} , Matthew Wook Chang _{1,

2}

Affiliation

Gram-positive Staphylococcus aureus infection that results in pneumonia, urinary tract infection, and in severe cases, sepsis, has recently been classified as a serious threat to public health. Rapid and cost-effective detection of these infections are costly and time-consuming. Here, we present probiotic lactic acid bacteria engineered to detect autoinducer peptide-I (AIP-I), a quorum sensing molecule produced by Staphylococcus sp. during pathogenesis. We achieved this by adapting the well-characterized agr quorum sensing (agrQS) from Staphylococcus aureus into Lactobacillus reuteri. The engineered biosensor is able to detect AIP-I levels in the nanomolar to micromolar range. We further investigated the function of the biosensor to detect real-time changes in AIP-I levels to understand the dynamics of Staphylococcus aureus under various strenuous conditions. The developed sensors would be useful for detection of Staphylococcus contamination in hospital settings and for high-throughput drug screening.

中文翻译：

重编程益生菌罗伊氏乳杆菌作为金黄色葡萄球菌衍生的AIP-I检测的生物传感器

革兰氏阳性金黄色葡萄球菌感染可导致肺炎，尿路感染，严重者可导致败血症，最近已被列为对公共健康的严重威胁。快速且经济有效地检测这些感染既昂贵又费时。在这里，我们介绍了被设计用来检测自身诱导物肽-I（AIP-I）（一种由葡萄球菌sp。产生的群体感应分子）的益生菌乳酸菌。在发病过程中。我们通过将金黄色葡萄球菌的特征明确的agri群体感应（agr QS）改编为罗伊氏乳杆菌来实现这一目标。经过设计的生物传感器能够检测纳摩尔至微摩尔范围内的AIP-1水平。我们进一步研究了生物传感器检测AIP-1水平实时变化的功能，以了解在各种剧烈条件下金黄色葡萄球菌的动态变化。开发的传感器将有助于检测医院环境中的葡萄球菌污染和高通量药物筛查。

更新日期：2018-04-13

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