Scaffold-free three-dimensional cell culturing using magnetic levitation,Biomaterials Science

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Scaffold-free three-dimensional cell culturing using magnetic levitation
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-04-12 00:00:00 , DOI: 10.1039/c8bm00122g
Esra Türker _{1,

2,

3,

4} , Nida Demirçak _{1,

2,

3,

4} , Ahu Arslan-Yildiz _{1,

2,

3,

4}

Affiliation

Three-dimensional (3D) cell culture has emerged as a pioneering methodology and is increasingly utilized for tissue engineering, 3D bioprinting, cancer model studies and drug development studies. The 3D cell culture methodology provides artificial and functional cellular constructs serving as a modular playground prior to animal model studies, which saves substantial efforts, time and experimental costs. The major drawback of current 3D cell culture methods is their dependency on biocompatible scaffolds, which often require tedious syntheses and fabrication steps. Herein, we report an easy-to-use methodology for the formation of scaffold-free 3D cell culture and cellular assembly via magnetic levitation in the presence of paramagnetic agents. To paramagnetize the cell culture environment, three different Gadolinium(III) chelates were utilized, which led to levitation and assembly of cells at a certain levitation height. The assembly and close interaction of cells at the levitation height where the magnetic force was equilibrated with gravitational force triggered the formation of complex 3D cellular structures. It was shown that Gd(III) chelates provided an optimal levitation that induced intercellular interactions in scaffold-free format without compromising cell viability. NIH 3T3 mouse fibroblasts and HCC827 non-small-cell lung cancer cells were evaluated via the magnetic levitation system, and the formation of 3D cell culture models was validated for both cell lines. Hereby, the developed magnetic levitation system holds promises for complex cellular assemblies and 3D cell culture studies.

中文翻译：

磁悬浮无支架三维细胞培养

三维（3D）细胞培养已成为一种开创性的方法，并且越来越多地用于组织工程，3D生物打印，癌症模型研究和药物开发研究。3D细胞培养方法学提供了人工和功能性细胞构建体，可作为动物模型研究之前的模块化游乐场，从而节省了大量工作，时间和实验成本。当前3D细胞培养方法的主要缺点是它们对生物相容性支架的依赖，这通常需要乏味的合成和制造步骤。在此，我们提出了一个易于使用的方法的形成无脚手架，3D细胞培养和细胞组装的经在顺磁性物质存在下进行磁悬浮。为了使细胞培养环境顺磁，利用了三种不同的Ga（III）螯合物，它们导致在一定的悬浮高度下悬浮和组装细胞。在重力与重力达到平衡的悬浮高度，细胞的组装和紧密相互作用触发了复杂的3D细胞结构的形成。结果表明，Gd（III）螯合物提供了一种最佳的悬浮作用，可以诱导无支架形式的细胞间相互作用，而不会损害细胞活力。通过以下方法评估了NIH 3T3小鼠成纤维细胞和HCC827非小细胞肺癌细胞磁悬浮系统，并验证了两种细胞系的3D细胞培养模型的形成。因此，开发的磁悬浮系统有望用于复杂的细胞组装和3D细胞培养研究。

更新日期：2018-04-12

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