Heat stroke is a lethal condition which can cause dysfunction in the central nervous system, multi-organ damage and even death. However, there is still limited knowledge of the detailed mechanism about the roles of lysosomes in heat stroke due to lack of effective tools. Herein, we introduce our previously developed hemicyanine with a large D–π–A structure as the key fluorophore to develop a new fluorescent probe (CPY) for ratiometric mapping of lysosomal pH changes in live cells under a heat shock stimulus.

中文翻译：

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一种基于半菁的比例荧光探针，用于绘制活细胞中暑期间的溶酶体pH †

中暑是致命的疾病，可能导致中枢神经系统功能障碍，多器官损伤甚至死亡。然而，由于缺乏有效的工具，关于溶酶体在中暑中的作用的详细机理仍然知之甚少。在这里，我们介绍我们以前开发的具有大D–π–A结构的半菁，作为开发新的荧光探针（CPY）的关键荧光团，该探针用于按比例绘制热休克刺激下活细胞的溶酶体pH变化。