Genetic manipulation of T cells is frequently inefficient, however, when combined with physical methods (i.e. electroporation) a promising alliance with immunotherapy can be formed. This study presents new data on permeabilization of murine thymocytes and splenocytes as a T cell model using pulsed electric (PEF) and electromagnetic field (EMF). The 300 ns, 500 ns, 2 μs and 100 μs pulse bursts in a broad range of PEF 0–8 kV/cm were applied separately and in combination with 3.3 T, 0.2 kV/cm EMF pulses. The permeabilization efficiency was evaluated using fluorescent dye (YO-PRO-1) and flow cytometry. It was shown that a >14% increase in thymocytes permeabilization is achieved when electroporation is applied in combination with EMF, however splenocytes responded in a different manner – a statistically significant (P < 0.05) reduction in permeabilization was observed. The cytokine secretion patterns were mainly unaltered independently on the applied treatment parameters determined by secretion of IFNγ, IL-4 and IL-17 – the main cytokines of Th1, Th2 and Th17 cells. The results of this study are useful for development of pulsed power protocols for effective genetic modification of T cells.

T细胞的遗传操作通常效率低下，但是，当与物理方法（即电穿孔）结合时，就可以与免疫疗法结成联盟。这项研究提供了有关使用脉冲电（PEF）和电磁场（EMF）作为T细胞模型的鼠胸腺细胞和脾细胞通透性的新数据。在广泛的PEF 0–8 kV / cm范围内分别应用300 ns，500 ns，2μs和100μs脉冲猝发，并与3.3 T，0.2 kV / cm的EMF脉冲组合使用。使用荧光染料（YO-PRO-1）和流式细胞仪评估通透效率。结果表明，将电穿孔与EMF联合应用可使胸腺细胞通透性增加> 14％，但脾细胞以不同的方式反应-统计学上显着（P <0。05）观察到通透性降低。细胞因子的分泌模式主要不受所应用的治疗参数的影响而改变，这些参数由IFNγ，IL-4和IL-17（Th1，Th2和Th17细胞的主要细胞因子）的分泌决定。这项研究的结果可用于开发脉冲功率协议，以有效地对T细胞进行遗传修饰。