In this study, the antiproliferative activity of peanut skin procyanidins (PSP) and six fractions (PSP-1∼6) isolated from PSP by several chromatographic steps on the human prostate cancer DU145 cells were evaluated. The results showed that PSP and PSP-1∼6 significantly inhibited the proliferation of DU145 cells. PSP-2 was the most effective fraction, which was identified as procyanidin B₃ mainly and procyanidin dimer [(E)C-luteolin or keampferol] secondarily. Moreover, the mechanism of antiproliferative activity of PSP-2 was investigated. It was observed that PSP-2 induced apoptotic cell death and cell cycle arrest at S phase in DU145 cells. PSP-2 caused the increase of intracellular ROS level and the decrease of Bcl-2/Bax ratio, and triggered the activation of p53 and caspases-3 in DU145 cells. Our findings demonstrated that procyanidins from peanut skin have the potential to be developed as an anti-prostate cancer agent.

在这项研究中，评估了花生皮原花青素（PSP）和通过几个色谱步骤从PSP分离出的六个级分（PSP-1〜6）对人前列腺癌DU145细胞的抗增殖活性。结果表明，PSP和PSP-1〜6显着抑制DU145细胞的增殖。PSP-2是最有效的组分，被鉴定为原花青素B ₃其次是原花青素二聚体[（E）C-木犀草素或keampferol]。此外，还研究了PSP-2的抗增殖活性机理。观察到PSP-2在DU145细胞中诱导凋亡细胞死亡和S期细胞周期停滞。PSP-2引起细胞内ROS水平升高，Bcl-2 / Bax比值降低，并触发DU145细胞中p53和caspases-3的活化。我们的发现表明，花生皮中的原花青素有可能被开发为抗前列腺癌药物。